biostudies-literatureOsborne MSNational Institute of Allergy and Infectious DiseasesNIAID NIH HHS534-548https://www.ebi.ac.uk/biostudies/studies/S-EPMC12016784biostudies-literatureUnknown122(4)YabG is a sporulation-specific protease that is conserved among sporulating bacteria. Clostridioides difficile YabG processes the cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat/exosporium proteins CotA and CdeM. In prior work that identified CspA as the co-germinant receptor, mutations in yabG were found which altered the co-germinants required to initiate spore germination. To understand how these mutations in the yabG locus contribute to C. difficile spore germination, we introduced these mutations into an isogenic background. Spores derived from C. difficile yabG_C207A (a catalytically inactive allele), C. difficile yabG_A46D, C. difficile yabG_G37E, and C. difficile yabG<sub>P153L</subMolecular microbiologyThe Impact of YabG Mutations on Clostridioides difficile Spore Germination and Processing of Spore Substrates.PMC12016784R01 AI1168955R01AI116895R01 AI1720435R01AI172043Sorg JABrehm JNHenriques AOOsborne MSOlivenca CCochran AMSerrano MfalseThe Impact of YabG Mutations on Clostridioides difficile Spore Germination and Processing of Spore Substrates.YabG is a sporulation-specific protease that is conserved among sporulating bacteria. Clostridioides difficile YabG processes the cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat/exosporium proteins CotA and CdeM. In prior work that identified CspA as the co-germinant receptor, mutations in yabG were found which altered the co-germinants required to initiate spore germination. To understand how these mutations in the yabG locus contribute to C. difficile spore germination, we introduced these mutations into an isogenic background. Spores derived from C. difficile yabG_C207A (a catalytically inactive allele), C. difficile yabG_A46D, C. difficile yabG_G37E, and C. difficile yabG<sub>P153L</sub2024-01-01T00:00:00Z2024 Oct2026-06-03T23:15:42.122Z2026-05-02T03:11:47.385ZS-EPMC120167843925842710.1111/mmi.15316