biostudies-literatureUnknown2023Baselli SLumpy skin disease (LSD) is a notifiable, transboundary cattle disease that spreads rapidly and has a relevant economic impact. The etiological agent is the LSD virus (LSDV), genus <i>Capripoxvirus</i>, and family <i>Poxviridae</i>. To date, LSDV is widely present in Africa, Asia, and in transcontinental regions like Russia, Turkey, and the Middle East, thus representing a continuous threat to free neighbours. Appropriate serosurveillance programs can complement disease control, inform about its spread, and enable the assessment of vaccination campaigns. Since reliable and practical diagnostic tools could improve serological surveillance, this study aimed to produce and characterize monoclonal antibodies (MAbs) that allowed us to develop ELISA tests for the serological detection of LSD. FoTransboundary and emerging diseases8378153https://www.ebi.ac.uk/biostudies/studies/S-EPMC12017155biostudies-literatureELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease.PMC12017155Brocchi ESabino MGrazioli SWolff JShtjefni VBaselli SPezzoni GHoffmann BCapucci LfalseELISA Methods Based on Monoclonal Antibodies for the Serological Diagnosis of Lumpy Skin Disease.Lumpy skin disease (LSD) is a notifiable, transboundary cattle disease that spreads rapidly and has a relevant economic impact. The etiological agent is the LSD virus (LSDV), genus <i>Capripoxvirus</i>, and family <i>Poxviridae</i>. To date, LSDV is widely present in Africa, Asia, and in transcontinental regions like Russia, Turkey, and the Middle East, thus representing a continuous threat to free neighbours. Appropriate serosurveillance programs can complement disease control, inform about its spread, and enable the assessment of vaccination campaigns. Since reliable and practical diagnostic tools could improve serological surveillance, this study aimed to produce and characterize monoclonal antibodies (MAbs) that allowed us to develop ELISA tests for the serological detection of LSD. Fo2023-01-01T00:00:00Z20232026-06-01T10:19:15.135Z2025-07-11T03:03:57.831ZS-EPMC120171554030367610.1155/2023/8378153