{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Dorbani I"],"pubmed_abstract":["<h4>Introduction</h4>Pulsed light (PL) is a non-thermal processing technology that inactivates microorganisms through high-intensity pulses of polychromatic light, including ultraviolet-C (UV-C). While the germicidal effect of PL has been widely studied, its impact on microbial proteomes remains poorly understood. Here, we investigate the proteomic response of <i>Bacillus pumilus</i> DSM492 (ATCC 27142) spores to PL treatment, comparing it to conventional UV-C 254 nm exposure.<h4>Methods</h4><i>B. pumilus</i> spores were either suspended in water or sprayed onto a polystyrene surface and exposed to PL or UV-C at fluences achieving a 5-log and a > 7-log reduction in viability. Proteomic changes were analyzed using mass spectrometry to identify proteins with decreased abundance after treatme"],"journal":["Frontiers in microbiology"],"pagination":["1579161"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12017682"],"repository":["biostudies-literature"],"pubmed_title":["UV-C and hydration state drive pulsed light-induced proteome damage in &lt;i&gt;Bacillus pumilus&lt;/i&gt; spores."],"pmcid":["PMC12017682"],"pubmed_authors":["Dorbani I","Armengaud J","Carlin F","Duport C"],"additional_accession":[]},"is_claimable":false,"name":"UV-C and hydration state drive pulsed light-induced proteome damage in &lt;i&gt;Bacillus pumilus&lt;/i&gt; spores.","description":"<h4>Introduction</h4>Pulsed light (PL) is a non-thermal processing technology that inactivates microorganisms through high-intensity pulses of polychromatic light, including ultraviolet-C (UV-C). While the germicidal effect of PL has been widely studied, its impact on microbial proteomes remains poorly understood. Here, we investigate the proteomic response of <i>Bacillus pumilus</i> DSM492 (ATCC 27142) spores to PL treatment, comparing it to conventional UV-C 254 nm exposure.<h4>Methods</h4><i>B. pumilus</i> spores were either suspended in water or sprayed onto a polystyrene surface and exposed to PL or UV-C at fluences achieving a 5-log and a > 7-log reduction in viability. Proteomic changes were analyzed using mass spectrometry to identify proteins with decreased abundance after treatme","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2025-07-02T03:04:47.957Z","creation":"2025-07-02T03:04:47.957Z"},"accession":"S-EPMC12017682","cross_references":{"pubmed":["40270826"],"doi":["10.3389/fmicb.2025.1579161"]}}