{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yin Y"],"funding":["NIGMS NIH HHS"],"pagination":["147-150"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12018070"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["580(7801)"],"pubmed_abstract":["Long noncoding RNAs (lncRNAs) and promoter- or enhancer-associated unstable transcripts locate preferentially to chromatin, where some regulate chromatin structure, transcription and RNA processing<sup>1-13</sup>. Although several RNA sequences responsible for nuclear localization have been identified-such as repeats in the lncRNA Xist and Alu-like elements in long RNAs<sup>14-16</sup>-how lncRNAs as a class are enriched at chromatin remains unknown. Here we describe a random, mutagenesis-coupled, high-throughput method that we name 'RNA elements for subcellular localization by sequencing' (mutREL-seq). Using this method, we discovered an RNA motif that recognizes the U1 small nuclear ribonucleoprotein (snRNP) and is essential for the localization of reporter RNAs to chromatin. Across the "],"journal":["Nature"],"pubmed_title":["U1 snRNP regulates chromatin retention of noncoding RNAs."],"pmcid":["PMC12018070"],"funding_grant_id":["R01 GM084089"],"pubmed_authors":["Yin Y","Shan G","Lu JY","Shao W","Zhang QC","Hong Y","Cui L","Zhang X","Tian B","Li P","Shen X","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"U1 snRNP regulates chromatin retention of noncoding RNAs.","description":"Long noncoding RNAs (lncRNAs) and promoter- or enhancer-associated unstable transcripts locate preferentially to chromatin, where some regulate chromatin structure, transcription and RNA processing<sup>1-13</sup>. Although several RNA sequences responsible for nuclear localization have been identified-such as repeats in the lncRNA Xist and Alu-like elements in long RNAs<sup>14-16</sup>-how lncRNAs as a class are enriched at chromatin remains unknown. Here we describe a random, mutagenesis-coupled, high-throughput method that we name 'RNA elements for subcellular localization by sequencing' (mutREL-seq). Using this method, we discovered an RNA motif that recognizes the U1 small nuclear ribonucleoprotein (snRNP) and is essential for the localization of reporter RNAs to chromatin. Across the ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Apr","modification":"2026-06-01T11:00:53.975Z","creation":"2025-07-09T03:04:51.883Z"},"accession":"S-EPMC12018070","cross_references":{"pubmed":["32238924"],"doi":["10.1038/s41586-020-2105-3"]}}