biostudies-literatureUnknown105(4)Lhotte RDonor/recipient mismatched HLA antigens can lead to the production of Donor-Specific Antibodies by the recipient, which are deleterious to organ transplants. The HLA-DQ locus is the most frequent target, with both the DQ beta and alpha chains involved. For deceased donors in particular, while HLA-DQB1 has been typed in emergencies for a long time, HLA-DQA1 has only recently been included. No imputation algorithmic tool was available to impute HLA-DQA1 until the development of HaploSFHI, trained on 61,393 two-field typings by NGS methods. We evaluated the accuracy of two-field HLA-DQA1 imputation from serological and two-field level HLA-A, B, DRB1, and DQB1 typings. We report a highly accurate two-field HLA-DQA1 prediction using a French test cohort of 7696 individuals, respectively reachinHLAe70197https://www.ebi.ac.uk/biostudies/studies/S-EPMC12018869biostudies-literatureEvaluating the Antigen and Eplet Accuracy of DQA1 Imputations With the HaploSFHI Two-Field HLA Typing Inference Tool.PMC12018869Lhotte RGatouillat GTafulo SToutirais OFrancois DFMoalic VWalencik AGuillaume NBasire ATurner DDautin GDubois VDormoy AMarie-Joelle ASFHI ConsortiumUsureau CAarnink ACroisille LDelbos FTop-Raymond ICongy NJollet ITaupin JLFilloux MRouzaire POMurphey CPedron BGuidicelli LGBernard GDevriese MBardy BLetort VProust BfalseEvaluating the Antigen and Eplet Accuracy of DQA1 Imputations With the HaploSFHI Two-Field HLA Typing Inference Tool.Donor/recipient mismatched HLA antigens can lead to the production of Donor-Specific Antibodies by the recipient, which are deleterious to organ transplants. The HLA-DQ locus is the most frequent target, with both the DQ beta and alpha chains involved. For deceased donors in particular, while HLA-DQB1 has been typed in emergencies for a long time, HLA-DQA1 has only recently been included. No imputation algorithmic tool was available to impute HLA-DQA1 until the development of HaploSFHI, trained on 61,393 two-field typings by NGS methods. We evaluated the accuracy of two-field HLA-DQA1 imputation from serological and two-field level HLA-A, B, DRB1, and DQB1 typings. We report a highly accurate two-field HLA-DQA1 prediction using a French test cohort of 7696 individuals, respectively reachin2025-01-01T00:00:00Z2025 Apr2025-07-10T03:08:56.295Z2025-07-10T03:08:56.295ZS-EPMC120188694026961410.1111/tan.70197