biostudies-literatureUnknown27Shanebeck KMNatural Sciences and Engineering Research Council of CanadaEnvironment and Climate Change CanadaToxoplasma gondii and Sarcocystis spp. are globally distributed coccidian parasites infecting endothermic vertebrates. Toxoplasma gondii is zoonotic, with widespread global prevalence in humans, domestic animals, and wildlife. Sarcocystis is a related and diverse genus, with species that use a range of definitive and intermediate hosts. In intermediate hosts, these tissue dwelling coccidians can be asymptomatic or cause disease through neural, hepatic, and transplacental infections. Semiaquatic mammals such as the North American river otter (Lontra canadensis) are at high risk of exposure to T. gondii and Sarcocystis spp. due to terrestrial runoff into freshwater environments. Their high trophic position and dual habitat use make them excellent sentinel species to monitor the presence of food and waterborne pathogens in ecosystems. Brain tissue was sampled from 89 river otters in Alberta, Canada. DNA of T. gondii was detected in 34 % of otters using magnetic capture sequence-specific DNA extraction and qPCR. Genotypes of T. gondii were identified using nested PCR and sequencing of the GRA6 and SAG2 genes, and included the most common clonal lineages in North America, Types I, II, and III, as well as Type-12 (X/A), which is highly pathogenic in sea otters. DNA of Sarcocystis spp. was detected in brain lysates of 30 % of otters via conventional PCR with primers targeting ITS1 and 18S ribosomal regions, and sequencing revealed S. lutrae and a species most closely related to, but distinct from, S. kitikmeotensis. This study suggests that river otters are exposed trophically to T. gondii shed by felids, and at least 2 species of Sarcocystis shed by unknown definitive hosts. Highly pathogenic S. neurona was not detected in this population, likely reflecting the absence of possum definitive hosts in northern Canada. The potential effects of T. gondii and Sarcocystis spp. on behaviour, health, and reproduction of river otters warrant further investigation.International journal for parasitology. Parasites and wildlife101069https://www.ebi.ac.uk/biostudies/studies/S-EPMC12019200biostudies-literatureDetection of Toxoplasma gondii (Types I, II, III and 12) and Sarcocystis spp. in the brains of river otter (Lontra canadensis) from Alberta, Canada.PMC12019200Hernandez-Ortiz ALagrue CShanebeck KMThomas PJMerks HJenkins EJDixon BRfalseDetection of Toxoplasma gondii (Types I, II, III and 12) and Sarcocystis spp. in the brains of river otter (Lontra canadensis) from Alberta, Canada.Toxoplasma gondii and Sarcocystis spp. are globally distributed coccidian parasites infecting endothermic vertebrates. Toxoplasma gondii is zoonotic, with widespread global prevalence in humans, domestic animals, and wildlife. Sarcocystis is a related and diverse genus, with species that use a range of definitive and intermediate hosts. In intermediate hosts, these tissue dwelling coccidians can be asymptomatic or cause disease through neural, hepatic, and transplacental infections. Semiaquatic mammals such as the North American river otter (Lontra canadensis) are at high risk of exposure to T. gondii and Sarcocystis spp. due to terrestrial runoff into freshwater environments. Their high trophic position and dual habitat use make them excellent sentinel species to monitor the presence of food and waterborne pathogens in ecosystems. Brain tissue was sampled from 89 river otters in Alberta, Canada. DNA of T. gondii was detected in 34 % of otters using magnetic capture sequence-specific DNA extraction and qPCR. Genotypes of T. gondii were identified using nested PCR and sequencing of the GRA6 and SAG2 genes, and included the most common clonal lineages in North America, Types I, II, and III, as well as Type-12 (X/A), which is highly pathogenic in sea otters. DNA of Sarcocystis spp. was detected in brain lysates of 30 % of otters via conventional PCR with primers targeting ITS1 and 18S ribosomal regions, and sequencing revealed S. lutrae and a species most closely related to, but distinct from, S. kitikmeotensis. This study suggests that river otters are exposed trophically to T. gondii shed by felids, and at least 2 species of Sarcocystis shed by unknown definitive hosts. Highly pathogenic S. neurona was not detected in this population, likely reflecting the absence of possum definitive hosts in northern Canada. The potential effects of T. gondii and Sarcocystis spp. on behaviour, health, and reproduction of river otters warrant further investigation.2025-01-01T00:00:00Z2025 Aug2026-06-01T10:31:07.246Z2025-07-12T03:04:25.678ZS-EPMC120192004027632510.1016/j.ijppaw.2025.101069