biostudies-literatureUnknown32Lai BQThe spinal cord's limited regeneration is attributed to the scarcity of endogenous stem cells and a poor post-injury microenvironment in adult mammals. To overcome these challenges, we transplanted a DNA aptamer 19S (Apt19S) sustained-release decellularized optic nerve (DON) scaffold (DON-A) into completely transected spinal cord injury (SCI) site in rats and investigated its effect on endogenous stem cell recruitment and differentiation, which subsequently contributed to <i>in situ</i> SCI repair. It has been demonstrated that Apt19S specifically binds to the membrane receptor alkaline phosphatase highly expressed on neural stem cells (NSCs) and mesenchymal stem cells (MSCs), and our study further proved that Apt19S can simultaneously recruit endogenous NSCs and MSCs to the lesion of SCI.Materials today. Bio101753https://www.ebi.ac.uk/biostudies/studies/S-EPMC12019207biostudies-literatureDON-Apt19S bioactive scaffold transplantation promotes <i>in situ</i> spinal cord repair in rats with transected spinal cord injury by effectively recruiting endogenous neural stem cells and mesenchymal stem cells.PMC12019207Yang SBYang YHZheng QJChen YFWu RJLiu RLai BQWu CRYu HYXu JFu GTHua NLi XYang YChen ZChen ZHChe MTWu TTGuo YNfalseDON-Apt19S bioactive scaffold transplantation promotes <i>in situ</i> spinal cord repair in rats with transected spinal cord injury by effectively recruiting endogenous neural stem cells and mesenchymal stem cells.The spinal cord's limited regeneration is attributed to the scarcity of endogenous stem cells and a poor post-injury microenvironment in adult mammals. To overcome these challenges, we transplanted a DNA aptamer 19S (Apt19S) sustained-release decellularized optic nerve (DON) scaffold (DON-A) into completely transected spinal cord injury (SCI) site in rats and investigated its effect on endogenous stem cell recruitment and differentiation, which subsequently contributed to <i>in situ</i> SCI repair. It has been demonstrated that Apt19S specifically binds to the membrane receptor alkaline phosphatase highly expressed on neural stem cells (NSCs) and mesenchymal stem cells (MSCs), and our study further proved that Apt19S can simultaneously recruit endogenous NSCs and MSCs to the lesion of SCI.2025-01-01T00:00:00Z2025 Jun2025-07-01T03:05:41.012Z2025-07-01T03:05:41.012ZS-EPMC120192074027596010.1016/j.mtbio.2025.101753