{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pedersen FB"],"funding":["Bloddonorernes Forskningsfond"],"pagination":["e70164"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12019580"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["105(4)"],"pubmed_abstract":["HLA typing of large donor registries and biobanks as well as acute single patient/donor samples remains expensive, slow and logistically challenging, despite recent developments in the field. We have tested and validated a cost-effective, accurate and highly scalable method for typing specific genes in the HLA region. This enables HLA typing from 1 to 96 individuals simultaneously, using a targeted PCR and Native Barcoding kit from Oxford Nanopore Technologies. A primer set for seven HLA genes (HLA-A, -B, -C, -DRB1, -DQA1, -DQB1 and -DPB1) was developed to work in a multiplex PCR reaction. The resulting amplicons provide a possible four-field resolution of the HLA Class I genes and G-group resolution of the HLA Class II genes. The entire process, from DNA to HLA typing result, takes a tota"],"journal":["HLA"],"pubmed_title":["Cost-Effective and Highly Scalable Typing of HLA Classes I and II Genes of up to 96 Individuals Using Nanopore Sequencing."],"pmcid":["PMC12019580"],"funding_grant_id":["FF 2022/01"],"pubmed_authors":["Bruunsgaard H","Pedersen FB","Hansen JF","Andersen LS","Blomsterberg S","Hauge AW"],"additional_accession":[]},"is_claimable":false,"name":"Cost-Effective and Highly Scalable Typing of HLA Classes I and II Genes of up to 96 Individuals Using Nanopore Sequencing.","description":"HLA typing of large donor registries and biobanks as well as acute single patient/donor samples remains expensive, slow and logistically challenging, despite recent developments in the field. We have tested and validated a cost-effective, accurate and highly scalable method for typing specific genes in the HLA region. This enables HLA typing from 1 to 96 individuals simultaneously, using a targeted PCR and Native Barcoding kit from Oxford Nanopore Technologies. A primer set for seven HLA genes (HLA-A, -B, -C, -DRB1, -DQA1, -DQB1 and -DPB1) was developed to work in a multiplex PCR reaction. The resulting amplicons provide a possible four-field resolution of the HLA Class I genes and G-group resolution of the HLA Class II genes. The entire process, from DNA to HLA typing result, takes a tota","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-07-01T03:05:31.748Z","creation":"2025-07-01T03:05:31.748Z"},"accession":"S-EPMC12019580","cross_references":{"pubmed":["40270210"],"doi":["10.1111/tan.70164"]}}