biostudies-literatureUnknown16Antunes AThe venom of <i>Cyriopagopus schmidti</i> spider has been extensively investigated, thereby allowing the identification of numerous new natural peptides. Many of these peptides are active on ion channels and several of them occur from post-translational processing. In order to further identify new entities, we screened this venom against five different human voltage-gated sodium (hNa<sub>v</sub>) channels. We illustrate the unusual richness of this venom in targeting this wide variety of hNa<sub>v</sub> channels. We confirm the identity of previously discovered peptides active on these ion channels type (huwentoxin (HwTx)-I, HwTx-II and HwTx-IV), indicating the efficacy of the screening process by automated patch-clamp. We also identified a novel analogue of HwTx-IV that differs by the absFrontiers in pharmacology1566312https://www.ebi.ac.uk/biostudies/studies/S-EPMC12019880biostudies-literatureThe venom of <i>Cyriopagopus schmidti</i> spider contains a natural huwentoxin-IV analogue with unexpected improved analgesic potential.PMC12019880De Waard MBenoit EServent DLopez LThomas BAntunes ARibeiro Oliveira-Mendes BJaquillard LKhakh KMontnach JCohen CJBeroud RfalseThe venom of <i>Cyriopagopus schmidti</i> spider contains a natural huwentoxin-IV analogue with unexpected improved analgesic potential.The venom of <i>Cyriopagopus schmidti</i> spider has been extensively investigated, thereby allowing the identification of numerous new natural peptides. Many of these peptides are active on ion channels and several of them occur from post-translational processing. In order to further identify new entities, we screened this venom against five different human voltage-gated sodium (hNa<sub>v</sub>) channels. We illustrate the unusual richness of this venom in targeting this wide variety of hNa<sub>v</sub> channels. We confirm the identity of previously discovered peptides active on these ion channels type (huwentoxin (HwTx)-I, HwTx-II and HwTx-IV), indicating the efficacy of the screening process by automated patch-clamp. We also identified a novel analogue of HwTx-IV that differs by the abs2025-01-01T00:00:00Z20252025-06-26T03:05:27.25Z2025-06-26T03:05:27.25ZS-EPMC120198804027661010.3389/fphar.2025.1566312