biostudies-literatureUnknown103Wang KCottonseed meal (CSM) is abundant in proteins that have the potential to substitute for conventionally utilized protein supplements for animals. However, the presence of anti-nutritional factors in CSM, particularly free gossypol, has limited its application. This study evaluated the nutritional value of a cottonseed protein isolate (CPI) derived from CSM using an alkaline extraction and acid precipitation process and explored its effect on intestinal health in growing pigs. 32 Duroc-Landrace-Yorkshire castrated male pigs (initial body weight 19 ± 2 kg) were divided into 4 treatment groups: nitrogen-free diet, corn-soybean meal diet (CSD), cottonseed meal diet (CSMD, where CSM replaced 35% of the nitrogen in CSD), CPI diet (CPID, where CPI replaced 35% of the nitrogen in CSD). Our study reJournal of animal scienceskaf057https://www.ebi.ac.uk/biostudies/studies/S-EPMC12019961biostudies-literatureImproved quality of cottonseed meal: effect of cottonseed protein isolate on growth performance, nutrient digestibility, and intestinal health in growing pigs.PMC12019961Wang QRen KHe JMao XLuo YZheng PYan HWang KfalseImproved quality of cottonseed meal: effect of cottonseed protein isolate on growth performance, nutrient digestibility, and intestinal health in growing pigs.Cottonseed meal (CSM) is abundant in proteins that have the potential to substitute for conventionally utilized protein supplements for animals. However, the presence of anti-nutritional factors in CSM, particularly free gossypol, has limited its application. This study evaluated the nutritional value of a cottonseed protein isolate (CPI) derived from CSM using an alkaline extraction and acid precipitation process and explored its effect on intestinal health in growing pigs. 32 Duroc-Landrace-Yorkshire castrated male pigs (initial body weight 19 ± 2 kg) were divided into 4 treatment groups: nitrogen-free diet, corn-soybean meal diet (CSD), cottonseed meal diet (CSMD, where CSM replaced 35% of the nitrogen in CSD), CPI diet (CPID, where CPI replaced 35% of the nitrogen in CSD). Our study re2025-01-01T00:00:00Z2025 Jan2025-06-26T03:05:36.281Z2025-06-26T03:05:36.281ZS-EPMC120199614003689710.1093/jas/skaf057