biostudies-literatureUnknown103Singh PThis study investigated the effects of up to 45% inclusion of whole pulse ingredients in grain-free (GF) diets on the excretion of bile acids (BAs) and other fecal metabolites in healthy large-breed dogs. Twenty-eight adult Siberian Huskies were fed 1 of 4 experimental diets formulated to meet the same macronutrient profiles for 20 wk: 1) grain-inclusive diet with 45% corn (Ctl), 2) GF diet with 15% pulses (Pulse15), 3) GF diet with 30% pulses (Pulse30), 4) GF diet with 45% pulses (Pulse45). All diets included chicken meal and pea starch. Fecal samples were collected on weeks 2 and 19. Bile acids were analyzed using ultra-performance liquid chromatography-MRM/MS technology, while fecal metabolites were analyzed using Agilent HP1000 high-performance liquid chromatography. Bile acids and fecJournal of animal scienceskaf075https://www.ebi.ac.uk/biostudies/studies/S-EPMC12019969biostudies-literatureWhole pulse ingredient inclusion in macronutrient-balanced diets increased fecal concentrations of propionic acid but not total bile acids in healthy adult large-breed dogs after 20 weeks.PMC12019969Singh PSaunders-Blades JSwanson KSSquires EJOba PMShoveller AKfalseWhole pulse ingredient inclusion in macronutrient-balanced diets increased fecal concentrations of propionic acid but not total bile acids in healthy adult large-breed dogs after 20 weeks.This study investigated the effects of up to 45% inclusion of whole pulse ingredients in grain-free (GF) diets on the excretion of bile acids (BAs) and other fecal metabolites in healthy large-breed dogs. Twenty-eight adult Siberian Huskies were fed 1 of 4 experimental diets formulated to meet the same macronutrient profiles for 20 wk: 1) grain-inclusive diet with 45% corn (Ctl), 2) GF diet with 15% pulses (Pulse15), 3) GF diet with 30% pulses (Pulse30), 4) GF diet with 45% pulses (Pulse45). All diets included chicken meal and pea starch. Fecal samples were collected on weeks 2 and 19. Bile acids were analyzed using ultra-performance liquid chromatography-MRM/MS technology, while fecal metabolites were analyzed using Agilent HP1000 high-performance liquid chromatography. Bile acids and fec2025-01-01T00:00:00Z2025 Jan2025-07-01T03:05:46.424Z2025-07-01T03:05:46.424ZS-EPMC120199694006563810.1093/jas/skaf075