biostudies-literatureZhao YKey Technologies Research and Development ProgramScience and Technology Development FundNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationSuzhou Applied Basic Research (Healthcare) Science and Technology Innovation ProgramNational Key Research and Development Program of Chinae2416330https://www.ebi.ac.uk/biostudies/studies/S-EPMC12021062biostudies-literatureUnknown12(16)Current diagnostics for sepsis-associated acute kidney injury (SA-AKI) detect kidney damage only at advanced stages, limiting opportunities for timely intervention. A DNA origami-based nanoplatform is developed for the early diagnosis and treatment of SA-AKI. Modified with a fluorophore (Cy5) and quencher (BHQ3), the DNA origami remains nonfluorescent under normal conditions. During SA-AKI, elevated microRNA-21 triggers a strand displacement reaction that restores the fluorescence signal, enabling real-time detection. Additionally, the photoacoustic changes of BHQ3, driven by different excretion rates of the nanostructure and released DNA strands, enable dual-mode imaging, enhancing diagnostic accuracy. Therapeutically, DNA origami scavenges reactive oxygen species and, when conjugated witAdvanced science (Weinheim, Baden-Wurttemberg, Germany)A Dual-Response DNA Origami Platform for Imaging and Treatment of Sepsis-Associated Acute Kidney Injury.PMC120210622024M762281SYW20240682023YFB32082000002/2022/AKP0115/2023/RIA2218254022207410122393932Dong FLing YHe YChen ZLu XWang HZhao YWu XfalseA Dual-Response DNA Origami Platform for Imaging and Treatment of Sepsis-Associated Acute Kidney Injury.Current diagnostics for sepsis-associated acute kidney injury (SA-AKI) detect kidney damage only at advanced stages, limiting opportunities for timely intervention. A DNA origami-based nanoplatform is developed for the early diagnosis and treatment of SA-AKI. Modified with a fluorophore (Cy5) and quencher (BHQ3), the DNA origami remains nonfluorescent under normal conditions. During SA-AKI, elevated microRNA-21 triggers a strand displacement reaction that restores the fluorescence signal, enabling real-time detection. Additionally, the photoacoustic changes of BHQ3, driven by different excretion rates of the nanostructure and released DNA strands, enable dual-mode imaging, enhancing diagnostic accuracy. Therapeutically, DNA origami scavenges reactive oxygen species and, when conjugated wit2025-01-01T00:00:00Z2025 Apr2026-05-29T17:11:57.536Z2025-06-26T03:05:27.268ZS-EPMC120210624001935710.1002/advs.202416330