biostudies-literatureUnknown19(4)Vera-Cabrera LActinomadura isolates obtained from seven human mycetoma cases in Mexico were characterized using nucleotide sequence analysis of a portion of the small subunit ribosomal RNA gene. Most isolates were identified as Actinomadura madurae. However, one isolate, LIID-AQ337, showed inconclusive results. To determine its identity, genomic DNA from LIID-AQ337 was subjected to whole-genome sequencing using both short- and long-read sequencing, resulting in a genome of 8,163,638-bp spread in 22 contigs. Comparative analysis against currently available genomes of other Actinomadura species suggested that LIID-AQ337 belongs to a new Actinomadura species. We propose to name it Actinomadura welshii sp. nov in honor of Oliverio Welsh, a Mexican dermatologist dedicated to mycetoma studies in Mexico.PLoS neglected tropical diseasese0013016https://www.ebi.ac.uk/biostudies/studies/S-EPMC12021271biostudies-literatureActinomadura welshii sp. nov., a New Mycetoma Agent in Mexico.PMC12021271Aguilera-Valenciano MAVera-Cabrera LMolina-Torres CACrane AEAvanzi CGallardo-Rocha AEscalante-Fuentes WGCantu-Alvarez MGOcampo-Candiani JfalseActinomadura welshii sp. nov., a New Mycetoma Agent in Mexico.Actinomadura isolates obtained from seven human mycetoma cases in Mexico were characterized using nucleotide sequence analysis of a portion of the small subunit ribosomal RNA gene. Most isolates were identified as Actinomadura madurae. However, one isolate, LIID-AQ337, showed inconclusive results. To determine its identity, genomic DNA from LIID-AQ337 was subjected to whole-genome sequencing using both short- and long-read sequencing, resulting in a genome of 8,163,638-bp spread in 22 contigs. Comparative analysis against currently available genomes of other Actinomadura species suggested that LIID-AQ337 belongs to a new Actinomadura species. We propose to name it Actinomadura welshii sp. nov in honor of Oliverio Welsh, a Mexican dermatologist dedicated to mycetoma studies in Mexico.2025-01-01T00:00:00Z2025 Apr2026-06-02T04:12:43.815Z2026-04-14T03:13:14.397ZS-EPMC120212714021525310.1371/journal.pntd.0013016