{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Richens JH"],"funding":["Cancer Research UK","Deutsche Forschungsgemeinschaft","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["e3003099"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12021295"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(4)"],"pubmed_abstract":["Understanding how specific secretory cargoes are targeted to distinct domains of the plasma membrane in epithelial cells requires analyzing the trafficking of post-Golgi vesicles to their sites of secretion. We used the RUSH (retention using selective hooks) system to synchronously release an apical cargo, Cadherin 99C (Cad99C), and a basolateral cargo, the ECM protein Nidogen, from the endoplasmic reticulum and followed their movements to the plasma membrane. We also developed an interactive vesicle tracking framework, MSP-tracker and viewer, that exploits developments in computer vision and deep learning to determine vesicle trajectories in a noisy environment without the need for extensive training data. MSP-tracker outperformed other tracking software in detecting and tracking post-Gol"],"journal":["PLoS biology"],"pubmed_title":["MSP-tracker: A versatile vesicle tracking software tool used to reveal the spatial control of polarized secretion in Drosophila epithelial cells."],"pmcid":["PMC12021295"],"funding_grant_id":["A24823","203144","203285","095927","SFB 1009","092096","207496","SFB 1348","080007","A14492","BB/P026486/1"],"pubmed_authors":["Richens JH","Butler R","Camelo C","Muschalik N","Glashauser J","Zenner HL","Rittscher J","Dmitrieva M","Luschnig S","Munro S","St Johnston D"],"additional_accession":[]},"is_claimable":false,"name":"MSP-tracker: A versatile vesicle tracking software tool used to reveal the spatial control of polarized secretion in Drosophila epithelial cells.","description":"Understanding how specific secretory cargoes are targeted to distinct domains of the plasma membrane in epithelial cells requires analyzing the trafficking of post-Golgi vesicles to their sites of secretion. We used the RUSH (retention using selective hooks) system to synchronously release an apical cargo, Cadherin 99C (Cad99C), and a basolateral cargo, the ECM protein Nidogen, from the endoplasmic reticulum and followed their movements to the plasma membrane. We also developed an interactive vesicle tracking framework, MSP-tracker and viewer, that exploits developments in computer vision and deep learning to determine vesicle trajectories in a noisy environment without the need for extensive training data. MSP-tracker outperformed other tracking software in detecting and tracking post-Gol","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-05-29T19:04:00.338Z","creation":"2025-08-12T03:04:48.289Z"},"accession":"S-EPMC12021295","cross_references":{"pubmed":["40208901"],"doi":["10.1371/journal.pbio.3003099"]}}