{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Ding S"],"pubmed_abstract":["Ribonuclease H (RNase H), a critical functional protein in replication and genome stability, is emerging as a crucial therapeutic target for various diseases, including immune disorders. We present a transcription-based hybrid probe, referred to as Hybprobe, and a CRISPR/Cas12a signal amplifier for the rapid, sensitive, and low-cost detection of RNase H activity. In this method, the RNA strand of the Hybprobe is specifically cleaved by RNase H, releasing a single-stranded DNA activator that facilitates recognition and cleavage by the Cas12a/crRNA complex, triggering signal amplification via Cas12a's trans-cleavage activity. The proposed method demonstrates ultra-high sensitivity, capable of detecting RNase H as low as 9.02 × 10<sup>-10</sup> U/μL, making it approximately 1,000 times more s"],"journal":["Frontiers in pharmacology"],"pagination":["1589150"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12021801"],"repository":["biostudies-literature"],"pubmed_title":["Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier."],"pmcid":["PMC12021801"],"pubmed_authors":["Li X","Tang Z","Ding S","Ren K","Yang M","Shi J","Wei Y"],"additional_accession":[]},"is_claimable":false,"name":"Ultrasensitive RNase H activity detection using the transcription-based hybrid probe and CRISPR/cas12a signal amplifier.","description":"Ribonuclease H (RNase H), a critical functional protein in replication and genome stability, is emerging as a crucial therapeutic target for various diseases, including immune disorders. We present a transcription-based hybrid probe, referred to as Hybprobe, and a CRISPR/Cas12a signal amplifier for the rapid, sensitive, and low-cost detection of RNase H activity. In this method, the RNA strand of the Hybprobe is specifically cleaved by RNase H, releasing a single-stranded DNA activator that facilitates recognition and cleavage by the Cas12a/crRNA complex, triggering signal amplification via Cas12a's trans-cleavage activity. The proposed method demonstrates ultra-high sensitivity, capable of detecting RNase H as low as 9.02 × 10<sup>-10</sup> U/μL, making it approximately 1,000 times more s","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2025-07-09T03:04:52.561Z","creation":"2025-07-09T03:04:52.561Z"},"accession":"S-EPMC12021801","cross_references":{"pubmed":["40290436"],"doi":["10.3389/fphar.2025.1589150"]}}