biostudies-literatureCaillens VARCANRWWCRLa Ligue contre le cancerInstitut National de la Santé et de la Recherche Médicale14392https://www.ebi.ac.uk/biostudies/studies/S-EPMC12022052biostudies-literatureUnknown15(1)T cell activation is critical for adaptive immunity, helping to protect the body from infection and tumors. A key step in this activation is signal transduction downstream of the T cell antigen receptor. This signaling involves several steps, with early ones occurring at the plasma membrane and others that occur later, after TCR internalization. The late steps in TCR signaling remain poorly understood. Since the TCR can signal after its internalization, we postulated that kinases abundantly expressed in T cells may regulate TCR signaling. This study focuses on two such enzymes: integrin-linked kinase (ILKs) and threonine-tyrosine kinase (TTKs), whose involvement in TCR signaling has not been previously studied. Using specific depletion of TTK and ILK by lentiviral shRNA, we show that in thScientific reportsIntegrin linked kinase and threonine tyrosine kinase modulate TCR signaling.PMC12022052dotationEndosignIDEAPhD fellowshipBoisel ENugue MSaveanu LCaillens VOuksel AEvnouchidou IfalseIntegrin linked kinase and threonine tyrosine kinase modulate TCR signaling.T cell activation is critical for adaptive immunity, helping to protect the body from infection and tumors. A key step in this activation is signal transduction downstream of the T cell antigen receptor. This signaling involves several steps, with early ones occurring at the plasma membrane and others that occur later, after TCR internalization. The late steps in TCR signaling remain poorly understood. Since the TCR can signal after its internalization, we postulated that kinases abundantly expressed in T cells may regulate TCR signaling. This study focuses on two such enzymes: integrin-linked kinase (ILKs) and threonine-tyrosine kinase (TTKs), whose involvement in TCR signaling has not been previously studied. Using specific depletion of TTK and ILK by lentiviral shRNA, we show that in th2025-01-01T00:00:00Z2025 Apr2025-06-26T03:05:24.377Z2025-06-26T03:05:24.377ZS-EPMC120220524027492910.1038/s41598-025-99331-y