{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Yoshinaga T"],"funding":["Shionogi & Co., Ltd."],"pubmed_abstract":["Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these studies focused on validating the air collection method, and there was no report on the development of a virus detection method. In this study, to detect viruses in air samples with greater sensitively than conventional detection methods, we utilized COPMAN, a highly sensitive virus detection method originally used for wastewater samples. We applied COPMAN to air samples, thereby developing COPMAN-Air. Briefly, this method efficiently detects the extremely low levels of viral RNA in air samples via three reaction steps: RT, preamplification, and qPCR, as it is performed with COPMAN. We evaluated COPMAN-Air using samples from a fever clinic for COVID-19 patients. COPMAN-Air demonstrated a higher det"],"journal":["Scientific reports"],"pagination":["14340"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12022069"],"repository":["biostudies-literature"],"pubmed_title":["Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air."],"pmcid":["PMC12022069"],"pubmed_authors":["Yoshinaga T","Kishida T","Ando Y","Sato Y","Kitajima M"],"additional_accession":[]},"is_claimable":false,"name":"Development of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air.","description":"Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these studies focused on validating the air collection method, and there was no report on the development of a virus detection method. In this study, to detect viruses in air samples with greater sensitively than conventional detection methods, we utilized COPMAN, a highly sensitive virus detection method originally used for wastewater samples. We applied COPMAN to air samples, thereby developing COPMAN-Air. Briefly, this method efficiently detects the extremely low levels of viral RNA in air samples via three reaction steps: RT, preamplification, and qPCR, as it is performed with COPMAN. We evaluated COPMAN-Air using samples from a fever clinic for COVID-19 patients. COPMAN-Air demonstrated a higher det","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-15T11:03:29.404Z","creation":"2026-07-03T03:15:51.102Z"},"accession":"S-EPMC12022069","cross_references":{"pubmed":["40275056"],"doi":["10.1038/s41598-025-99365-2"]}}