biostudies-literatureUnknown15(1)Yoshinaga TShionogi & Co., Ltd.Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these studies focused on validating the air collection method, and there was no report on the development of a virus detection method. In this study, to detect viruses in air samples with greater sensitively than conventional detection methods, we utilized COPMAN, a highly sensitive virus detection method originally used for wastewater samples. We applied COPMAN to air samples, thereby developing COPMAN-Air. Briefly, this method efficiently detects the extremely low levels of viral RNA in air samples via three reaction steps: RT, preamplification, and qPCR, as it is performed with COPMAN. We evaluated COPMAN-Air using samples from a fever clinic for COVID-19 patients. COPMAN-Air demonstrated a higher detScientific reports14340https://www.ebi.ac.uk/biostudies/studies/S-EPMC12022069biostudies-literatureDevelopment of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air.PMC12022069Yoshinaga TKishida TAndo YSato YKitajima MfalseDevelopment of COPMAN-Air method for high-sensitivity detection of SARS-CoV-2 in air.Several studies have successfully detected SARS-CoV-2 in air samples. However, most of these studies focused on validating the air collection method, and there was no report on the development of a virus detection method. In this study, to detect viruses in air samples with greater sensitively than conventional detection methods, we utilized COPMAN, a highly sensitive virus detection method originally used for wastewater samples. We applied COPMAN to air samples, thereby developing COPMAN-Air. Briefly, this method efficiently detects the extremely low levels of viral RNA in air samples via three reaction steps: RT, preamplification, and qPCR, as it is performed with COPMAN. We evaluated COPMAN-Air using samples from a fever clinic for COVID-19 patients. COPMAN-Air demonstrated a higher det2025-01-01T00:00:00Z2025 Apr2026-07-15T11:03:29.404Z2026-07-03T03:15:51.102ZS-EPMC120220694027505610.1038/s41598-025-99365-2