biostudies-literatureLi Jhouqiang xuGuizhou University1101https://www.ebi.ac.uk/biostudies/studies/S-EPMC12023973biostudies-literatureUnknown15(8)The <i>DHRS3</i> gene, a member of the short-chain dehydrogenase/reductase (SDR) family, is involved in critical metabolic processes in animals. This study investigated the expression patterns of <i>DHRS3</i> across various tissues of developmental stages in pigs and preliminarily evaluated its effects on myoblast proliferation, apoptosis, and differentiation. RT-qPCR (real-time quantitative PCR) was employed to analyze <i>DHRS3</i> expression in the heart, liver, spleen, lungs, kidneys, longissimus dorsi, foreleg, and hind leg of pigs at 3 days, 6 months, and 12 months of age. Cell proliferation was analyzed using EdU (5-ethynyl-2'-deoxyuridine) assays, RT-qPCR, and flow cytometry, while the expression changes of proliferation-, apoptosis-, and differentiation-related genes were assessed Animals : an open access journal from MDPITissue-Specific Expression of the Porcine <i>DHRS3</i> Gene and Its Impact on the Proliferation and Differentiation of Myogenic Cells.PMC12023973QKHFQ-2018,4007, (002)QKHKYZ-2019,011Liu HRuan YLi JLi ZZhou BJiang CXu HSun JAn DfalseTissue-Specific Expression of the Porcine <i>DHRS3</i> Gene and Its Impact on the Proliferation and Differentiation of Myogenic Cells.The <i>DHRS3</i> gene, a member of the short-chain dehydrogenase/reductase (SDR) family, is involved in critical metabolic processes in animals. This study investigated the expression patterns of <i>DHRS3</i> across various tissues of developmental stages in pigs and preliminarily evaluated its effects on myoblast proliferation, apoptosis, and differentiation. RT-qPCR (real-time quantitative PCR) was employed to analyze <i>DHRS3</i> expression in the heart, liver, spleen, lungs, kidneys, longissimus dorsi, foreleg, and hind leg of pigs at 3 days, 6 months, and 12 months of age. Cell proliferation was analyzed using EdU (5-ethynyl-2'-deoxyuridine) assays, RT-qPCR, and flow cytometry, while the expression changes of proliferation-, apoptosis-, and differentiation-related genes were assessed 2025-01-01T00:00:00Z2025 Apr2026-05-23T03:14:37.703Z2025-07-07T03:08:52.428ZS-EPMC120239734028193510.3390/ani15081101