{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nayyab S"],"funding":["NIH HHS"],"pagination":["601"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12024862"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(4)"],"pubmed_abstract":["The testis-specific serine kinases (TSSKs) are post-meiotically expressed in testicular germ cells. Their testis-specific expression, together with their putative role in phosphorylation pathways, suggests that TSSKs have relevant roles in spermiogenesis, sperm function, or both. Independent <i>Tssk3</i> and <i>Tssk6</i> knockout mice, as well as the double <i>Tssk1</i>/<i>Tssk2</i> KO males, are sterile. However, the double KO results are silent regarding the individual roles of TSSK1 and TSSK2. The aim of this study was to develop independent mutant mouse models of <i>Tssk1</i> and <i>Tssk2</i>, using CRISPR/Cas9, to evaluate their independent roles in reproduction. Male heterozygous pups were used to establish one <i>Tssk1</i> and two independent <i>Tssk2</i> mutant lines. Natural matin"],"journal":["Biomolecules"],"pubmed_title":["Identification of TSSK1 and TSSK2 as Novel Targets for Male Contraception."],"pmcid":["PMC12024862"],"funding_grant_id":["1R01HD038082-21A1"],"pubmed_authors":["Akizawa H","Snyder E","Georg GI","Shamailova Y","Cui W","Gervasi MG","Salicioni AM","Fissore R","Taghavi M","Nayyab S","Tourzani DA","Visconti PE"],"additional_accession":[]},"is_claimable":false,"name":"Identification of TSSK1 and TSSK2 as Novel Targets for Male Contraception.","description":"The testis-specific serine kinases (TSSKs) are post-meiotically expressed in testicular germ cells. Their testis-specific expression, together with their putative role in phosphorylation pathways, suggests that TSSKs have relevant roles in spermiogenesis, sperm function, or both. Independent <i>Tssk3</i> and <i>Tssk6</i> knockout mice, as well as the double <i>Tssk1</i>/<i>Tssk2</i> KO males, are sterile. However, the double KO results are silent regarding the individual roles of TSSK1 and TSSK2. The aim of this study was to develop independent mutant mouse models of <i>Tssk1</i> and <i>Tssk2</i>, using CRISPR/Cas9, to evaluate their independent roles in reproduction. Male heterozygous pups were used to establish one <i>Tssk1</i> and two independent <i>Tssk2</i> mutant lines. Natural matin","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-03T03:24:14.121Z","creation":"2025-07-08T03:12:46.486Z"},"accession":"S-EPMC12024862","cross_references":{"pubmed":["40305308"],"doi":["10.3390/biom15040601"]}}