biostudies-literatureNayyab SNIH HHS601https://www.ebi.ac.uk/biostudies/studies/S-EPMC12024862biostudies-literatureUnknown15(4)The testis-specific serine kinases (TSSKs) are post-meiotically expressed in testicular germ cells. Their testis-specific expression, together with their putative role in phosphorylation pathways, suggests that TSSKs have relevant roles in spermiogenesis, sperm function, or both. Independent Tssk3 and Tssk6 knockout mice, as well as the double Tssk1/Tssk2 KO males, are sterile. However, the double KO results are silent regarding the individual roles of TSSK1 and TSSK2. The aim of this study was to develop independent mutant mouse models of Tssk1 and Tssk2, using CRISPR/Cas9, to evaluate their independent roles in reproduction. Male heterozygous pups were used to establish one Tssk1 and two independent Tssk2 mutant lines. Natural matinBiomoleculesIdentification of TSSK1 and TSSK2 as Novel Targets for Male Contraception.PMC120248621R01HD038082-21A1Akizawa HSnyder EGeorg GIShamailova YCui WGervasi MGSalicioni AMFissore RTaghavi MNayyab STourzani DAVisconti PEfalseIdentification of TSSK1 and TSSK2 as Novel Targets for Male Contraception.The testis-specific serine kinases (TSSKs) are post-meiotically expressed in testicular germ cells. Their testis-specific expression, together with their putative role in phosphorylation pathways, suggests that TSSKs have relevant roles in spermiogenesis, sperm function, or both. Independent Tssk3 and Tssk6 knockout mice, as well as the double Tssk1/Tssk2 KO males, are sterile. However, the double KO results are silent regarding the individual roles of TSSK1 and TSSK2. The aim of this study was to develop independent mutant mouse models of Tssk1 and Tssk2, using CRISPR/Cas9, to evaluate their independent roles in reproduction. Male heterozygous pups were used to establish one Tssk1 and two independent Tssk2 mutant lines. Natural matin2025-01-01T00:00:00Z2025 Apr2026-07-03T03:24:14.121Z2025-07-08T03:12:46.486ZS-EPMC120248624030530810.3390/biom15040601