{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang RX"],"funding":["National Natural Science Foundation of China"],"pagination":["3796"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12028029"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(8)"],"pubmed_abstract":["Hexaploid wheat has a large genome, making it difficult for transgenes to produce phenotypes due to gene redundancy and tight linkage among genes. Multiple gene copies typically necessitate multiple targeting events during gene editing, followed by several generations of self-crossing to achieve homozygous genotypes. The high cost of transgenesis in wheat is another issue, which hinders the easy availability of gene-edited materials in wheat. In this study, we developed a comprehensive approach to improve wheat gene editing efficiency. First, we established a protoplast-based system to evaluate the relative efficiency of gene editing targets, which enabled the rapid and effective selection of optimal sgRNAs. We then compared two transformation strategies: biolistic bombardment and Agrobact"],"journal":["International journal of molecular sciences"],"pubmed_title":["An Optimized Editing Approach for Wheat Genes by Improving sgRNA Design and Transformation Strategies."],"pmcid":["PMC12028029"],"funding_grant_id":["32270372"],"pubmed_authors":["Yang H","Sun WH","Yang ZG","Yang Z","Zhang YF","Zhang XD","Liu WT","Li BB","Zhang RX","Chen KM"],"additional_accession":[]},"is_claimable":false,"name":"An Optimized Editing Approach for Wheat Genes by Improving sgRNA Design and Transformation Strategies.","description":"Hexaploid wheat has a large genome, making it difficult for transgenes to produce phenotypes due to gene redundancy and tight linkage among genes. Multiple gene copies typically necessitate multiple targeting events during gene editing, followed by several generations of self-crossing to achieve homozygous genotypes. The high cost of transgenesis in wheat is another issue, which hinders the easy availability of gene-edited materials in wheat. In this study, we developed a comprehensive approach to improve wheat gene editing efficiency. First, we established a protoplast-based system to evaluate the relative efficiency of gene editing targets, which enabled the rapid and effective selection of optimal sgRNAs. We then compared two transformation strategies: biolistic bombardment and Agrobact","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-04-08T19:22:28.066Z","creation":"2026-04-08T13:21:21.232Z"},"accession":"S-EPMC12028029","cross_references":{"pubmed":["40332465"],"doi":["10.3390/ijms26083796"]}}