biostudies-literatureUnknown13(4)Yao LMangrove forests are intertidal ecosystems that harbor diverse microbial communities essential for biogeochemical cycles and energy flow. This study investigated the seasonal and spatial patterns of bacterial communities in the artificially introduced mangrove sediments of the Ao River estuary using 16S rRNA gene amplicon high-throughput sequencing. Alpha diversity analyses indicated that the bacterial community diversity in the mangrove sediments of the Ao River estuary was similar to those of natural-formed mangroves, with the Shannon index ranging from 5.16 to 6.54, which was significantly higher in winter compared to other seasons. The dominant bacterial phyla included Proteobacteria (43.65%), Actinobacteria (11.55%), Desulfobacterota (11.16%), and Bacteroidetes (5.52%), while beta divMicroorganisms859https://www.ebi.ac.uk/biostudies/studies/S-EPMC12029902biostudies-literatureSpatiotemporal Characteristics of Bacterial Communities in Estuarine Mangrove Sediments in Zhejiang Province, China.PMC12029902Yao LLi XHe MShui BJiang SfalseSpatiotemporal Characteristics of Bacterial Communities in Estuarine Mangrove Sediments in Zhejiang Province, China.Mangrove forests are intertidal ecosystems that harbor diverse microbial communities essential for biogeochemical cycles and energy flow. This study investigated the seasonal and spatial patterns of bacterial communities in the artificially introduced mangrove sediments of the Ao River estuary using 16S rRNA gene amplicon high-throughput sequencing. Alpha diversity analyses indicated that the bacterial community diversity in the mangrove sediments of the Ao River estuary was similar to those of natural-formed mangroves, with the Shannon index ranging from 5.16 to 6.54, which was significantly higher in winter compared to other seasons. The dominant bacterial phyla included Proteobacteria (43.65%), Actinobacteria (11.55%), Desulfobacterota (11.16%), and Bacteroidetes (5.52%), while beta div2025-01-01T00:00:00Z2025 Apr2026-04-07T21:24:15.926Z2025-07-07T03:08:10.824ZS-EPMC120299024028469610.3390/microorganisms13040859