{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ghafoor H"],"funding":["Pakistan Science Foundation"],"pagination":["36"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12031827"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["36(1)"],"pubmed_abstract":["Surgical Site infections (SSIs) affect up to 5% of surgical procedures, posing a significant postoperative complication. This study aims to develop and evaluate antibacterial clove/gelatin-coated sutures to reduce SSI infection. Clove extract, known for its antimicrobial properties, was incorporated into a gelatin matrix as a biocompatible coating for silk-braided sutures. Sutures were dip-coated in clove/gelatin solutions at concentrations of 5, 10, 15, and 20%. The surface morphology, chemical composition, and mechanical strength of the coated suture were characterized by using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and tensile testing. Antimicrobial efficacy was assessed via zone-of-inhibition assays against (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis). The 20% Clove/gelatin coating exhibited the highest antibacterial activity (17.5 ± 0.875 mm) and demonstrated low cytotoxicity in MTT assay with human primary dermal fibroblast. Hemolytic assays revealed that all composite concentrations resulted in <1% hemolytic activity. Approximately 93% of drug release was observed in 20% formulation within 72 h which is promising results compared with untreated sutures. Based on its superior antibacterial performance, the 20% formulation was selected for in vivo studies. In a rat abdominal incision model, wounds closed with coated sutures exhibited complete healing, while those closed with uncoated sutures remained partially healed. These findings highlight the potential of clove/gelatin-coated sutures for enhanced antimicrobial protection and improved wound healing in surgical applications."],"journal":["Journal of materials science. Materials in medicine"],"pubmed_title":["Synthesis and characterization of clove/gelatin coated silk sutures for surgical site infection and wound healing."],"pmcid":["PMC12031827"],"funding_grant_id":["PSF grant (PSF/Res/C-NUST/Med 521)."],"pubmed_authors":["Ghafoor H","Butt MS"],"additional_accession":[]},"is_claimable":false,"name":"Synthesis and characterization of clove/gelatin coated silk sutures for surgical site infection and wound healing.","description":"Surgical Site infections (SSIs) affect up to 5% of surgical procedures, posing a significant postoperative complication. This study aims to develop and evaluate antibacterial clove/gelatin-coated sutures to reduce SSI infection. Clove extract, known for its antimicrobial properties, was incorporated into a gelatin matrix as a biocompatible coating for silk-braided sutures. Sutures were dip-coated in clove/gelatin solutions at concentrations of 5, 10, 15, and 20%. The surface morphology, chemical composition, and mechanical strength of the coated suture were characterized by using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and tensile testing. Antimicrobial efficacy was assessed via zone-of-inhibition assays against (Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, and Enterococcus faecalis). The 20% Clove/gelatin coating exhibited the highest antibacterial activity (17.5 ± 0.875 mm) and demonstrated low cytotoxicity in MTT assay with human primary dermal fibroblast. Hemolytic assays revealed that all composite concentrations resulted in <1% hemolytic activity. Approximately 93% of drug release was observed in 20% formulation within 72 h which is promising results compared with untreated sutures. Based on its superior antibacterial performance, the 20% formulation was selected for in vivo studies. In a rat abdominal incision model, wounds closed with coated sutures exhibited complete healing, while those closed with uncoated sutures remained partially healed. These findings highlight the potential of clove/gelatin-coated sutures for enhanced antimicrobial protection and improved wound healing in surgical applications.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-06-06T17:40:12.02Z","creation":"2026-06-03T03:10:34.186Z"},"accession":"S-EPMC12031827","cross_references":{"pubmed":["40278944"],"doi":["10.1007/s10856-025-06886-3"]}}