biostudies-literatureChen YGuangxi Medical University Undergraduate Education and Teaching Reform ProjectInnovation Project of Guangxi Graduate EducationFuture Academic Star of Guangxi Medical UniversityYouth Science Foundation of Guangxi Medical UniversityChina Undergraduate Innovation and Entrepreneurship Training ProgramGuangxi Zhuang Autonomous Region Health Commission Scientific Research ProjectGuangxi Higher Education Undergraduate Teaching Reform ProjectThe First Affiliated Hospital of Guangxi Medical University Provincial and Ministerial Key Laboratory Cultivation ProjectGuangxi Educational Science Planning Key Project10732748251330059https://www.ebi.ac.uk/biostudies/studies/S-EPMC12033653biostudies-literatureUnknown32BackgroundAnaphase-promoting complex subunit 1 (ANAPC1) is a regulator of cellular mitosis and an important factor in tumorigenesis. To date, a comprehensive assessment of the potential role, biological behaviours, and clinical significance of ANAPC1 in colorectal cancer (CRC) is still lacking.Materials and methodsThis study integrated 2329 mRNA expression data, single-cell RNA sequencing (scRNA-seq), and internal immunohistochemistry of 416 tissue samples to comprehensively evaluate the abnormal expression pattern of ANAPC1 in CRC. It also incorporated evidence from immune infiltration analysis, functional enrichment analysis, and weighted gene co-expression network analysis to explore the biological behaviour of ANAPC1 in CRC. In addition, in vitro cell biology experiments such as real-tCancer control : journal of the Moffitt Cancer CenterThe Potential Biological Roles and Clinical Significance of Anaphase-Promoting Complex Subunit 1 in Colorectal Cancer.PMC12033653Z-A20220465JGY20230682022JGA1462023Z10WLXSZX231142022ZJY2791GXMUYSF202423Z-A20220415Z-A20210442No. 21-220-18S202310598169Li HTang YLHe RQChen YZChen GZeng DTHuang ZGZhan YTLi DMTang YXWen JYWei QYChen YfalseThe Potential Biological Roles and Clinical Significance of Anaphase-Promoting Complex Subunit 1 in Colorectal Cancer.BackgroundAnaphase-promoting complex subunit 1 (ANAPC1) is a regulator of cellular mitosis and an important factor in tumorigenesis. To date, a comprehensive assessment of the potential role, biological behaviours, and clinical significance of ANAPC1 in colorectal cancer (CRC) is still lacking.Materials and methodsThis study integrated 2329 mRNA expression data, single-cell RNA sequencing (scRNA-seq), and internal immunohistochemistry of 416 tissue samples to comprehensively evaluate the abnormal expression pattern of ANAPC1 in CRC. It also incorporated evidence from immune infiltration analysis, functional enrichment analysis, and weighted gene co-expression network analysis to explore the biological behaviour of ANAPC1 in CRC. In addition, in vitro cell biology experiments such as real-t2025-01-01T00:00:00Z2025 Jan-Dec2026-06-01T19:10:42.175Z2025-07-11T03:03:37.942ZS-EPMC120336534022994610.1177/10732748251330059