biostudies-literatureShi YScience and Technology Bureau of Jiaxing CityNatural Science Foundation of Zhejiang ProvinceZhejiang Provincial Health Bureau Science FoundationMedical Science and technology development Foundation，Nanjing Department of HealthNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaNatural Science Foundation of Jiangsu Provincee70315https://www.ebi.ac.uk/biostudies/studies/S-EPMC12034574biostudies-literatureUnknown15(5)<h4>Background</h4>Septic cardiomyopathy is a frequent complication in patients with sepsis and is associated with a high mortality rate. Given its clinical significance, understanding the precise underlying mechanism is of great value.<h4>Methods and results</h4>Our results unveiled that Z-DNA binding protein 1 (ZBP1) is upregulated in myocardial tissues of lipopolysaccharide (LPS)-treated mice. Single-cell mRNA sequencing (scRNA-seq) and single-nucleus mRNA sequencing (snRNA-seq) indicated that Zbp1 mRNA in endothelial cells, fibroblasts and macrophages appeared to be elevated by LPS, which is partially consistent with the results of immunofluorescence. Through echocardiography, we identified that global deletion of ZBP1 improves cardiac dysfunction and the survival rate of LPS-treated mClinical and translational medicineMyeloid deficiency of Z-DNA binding protein 1 restricts septic cardiomyopathy via promoting macrophage polarisation towards the M2-subtype.PMC12034574824003192023AZ31003YKK240672024KY442BK202402262022YFA1205904ZCLMS25H150382470384Ni JDu YHe LZhou YShi YWang LLi YTan XXu BSun SHan JXu XYu XKang LfalseMyeloid deficiency of Z-DNA binding protein 1 restricts septic cardiomyopathy via promoting macrophage polarisation towards the M2-subtype.<h4>Background</h4>Septic cardiomyopathy is a frequent complication in patients with sepsis and is associated with a high mortality rate. Given its clinical significance, understanding the precise underlying mechanism is of great value.<h4>Methods and results</h4>Our results unveiled that Z-DNA binding protein 1 (ZBP1) is upregulated in myocardial tissues of lipopolysaccharide (LPS)-treated mice. Single-cell mRNA sequencing (scRNA-seq) and single-nucleus mRNA sequencing (snRNA-seq) indicated that Zbp1 mRNA in endothelial cells, fibroblasts and macrophages appeared to be elevated by LPS, which is partially consistent with the results of immunofluorescence. Through echocardiography, we identified that global deletion of ZBP1 improves cardiac dysfunction and the survival rate of LPS-treated m2025-01-01T00:00:00Z2025 May2026-06-01T14:51:52.128Z2025-07-06T03:05:34.788ZS-EPMC120345744028934510.1002/ctm2.70315