biostudies-literatureUnknown15(1)Vajrychova MPreterm prelabour rupture of membranes (PPROM) complicated by intra-amniotic inflammation (IAI) represents a substantial proportion of preterm birth cases. Currently, IAI is frequently defined as amniotic fluid IL-6 concentration above 2,600 pg/mL. However, the amniotic fluid IL-6 concentration was never correlated with the global response of other proinflammatory proteins to the ongoing IAI. In this cross-sectional study, protein quantification was performed using mass spectrometry (MS) analysis followed by target quantification of selected proinflammatory proteins. Levels of amniotic fluid proteins determined by MS were put into the correlation with IL-6 concentration determined by electrochemiluminescence immunoassay method (ECLIA). In total, 925 proteins were efficiently quantified andScientific reports14814https://www.ebi.ac.uk/biostudies/studies/S-EPMC12038036biostudies-literatureQuantification of intra-amniotic inflammation in late preterm prelabour rupture of membranes.PMC12038036Bolehovska RVajrychova MKupcik RKukla RKacerovsky MSadibolova MStranik JfalseQuantification of intra-amniotic inflammation in late preterm prelabour rupture of membranes.Preterm prelabour rupture of membranes (PPROM) complicated by intra-amniotic inflammation (IAI) represents a substantial proportion of preterm birth cases. Currently, IAI is frequently defined as amniotic fluid IL-6 concentration above 2,600 pg/mL. However, the amniotic fluid IL-6 concentration was never correlated with the global response of other proinflammatory proteins to the ongoing IAI. In this cross-sectional study, protein quantification was performed using mass spectrometry (MS) analysis followed by target quantification of selected proinflammatory proteins. Levels of amniotic fluid proteins determined by MS were put into the correlation with IL-6 concentration determined by electrochemiluminescence immunoassay method (ECLIA). In total, 925 proteins were efficiently quantified and2025-01-01T00:00:00Z2025 Apr2025-07-04T03:06:01.228Z2025-07-04T03:06:01.228ZS-EPMC120380364029564710.1038/s41598-025-99969-8