{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ahmad H"],"funding":["American Heart Association","Ludwig Center for Cancer Stem Cell Research at Stanford University","NIA NIH HHS","Transatlantic Fondation Leducq Network of Excellence","NHLBI NIH HHS","Burroughs Wellcome Foundation Career Award for Medical Scientists","Young Scientist Programme","DZHK","National Institutes of Aging","NIH HHS"],"pagination":["396-404"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12038232"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(3)"],"pubmed_abstract":["<h4>Aims</h4>Single-cell RNA sequencing (scRNA-seq) is a powerful method for exploring the cellular heterogeneity within human atheroma but typically requires fresh tissue to preserve cell membrane integrity, limiting the feasibility of large-scale biobanking for later analysis. The aim of this study was to determine whether cryopreservation of fragile and necrotic atheroma tissue affects the viability and transcriptomic profiles of haematopoietic cells in subsequent scRNA-seq analysis, enabling the use of cryopreserved atheroma samples for future research.<h4>Methods and results</h4>We performed scRNA-seq on five paired fresh and cryopreserved atheroma samples-three from coronary arteries and two from carotid arteries. Each sample was enzymatically digested, sorted for CD45+ haematopoieti"],"journal":["Cardiovascular research"],"pubmed_title":["Single cell RNA sequencing of haematopoietic cells in fresh and frozen human atheroma tissue."],"pmcid":["PMC12038232"],"funding_grant_id":["DP2 HL157540","R01 AG086072","181PA34170022","R01 AG088657","R01 AG088656","R03AG06182","DP2-HL157540"],"pubmed_authors":["Nachun DC","Ma L","Ahmad H","Koyano T","Gopakumar J","Jaiswal S","Boyd J","Fong R","Huang X","D'Addabbo J","Nguyen PK","Woo YJ","Aalami O"],"additional_accession":[]},"is_claimable":false,"name":"Single cell RNA sequencing of haematopoietic cells in fresh and frozen human atheroma tissue.","description":"<h4>Aims</h4>Single-cell RNA sequencing (scRNA-seq) is a powerful method for exploring the cellular heterogeneity within human atheroma but typically requires fresh tissue to preserve cell membrane integrity, limiting the feasibility of large-scale biobanking for later analysis. The aim of this study was to determine whether cryopreservation of fragile and necrotic atheroma tissue affects the viability and transcriptomic profiles of haematopoietic cells in subsequent scRNA-seq analysis, enabling the use of cryopreserved atheroma samples for future research.<h4>Methods and results</h4>We performed scRNA-seq on five paired fresh and cryopreserved atheroma samples-three from coronary arteries and two from carotid arteries. Each sample was enzymatically digested, sorted for CD45+ haematopoieti","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-05T03:09:41.008Z","creation":"2026-07-05T03:08:12.586Z"},"accession":"S-EPMC12038232","cross_references":{"pubmed":["39907372"],"doi":["10.1093/cvr/cvaf014"]}}