biostudies-literatureChen YGuangxi Medical University Undergraduate Education and Teaching Reform ProjectNatural Science Foundation of Guangxi ProvinceFuture Academic Star of Guangxi Medical UniversityChina Undergraduate Innovation and Entrepreneurship Training ProgramGuangxi Higher Education Undergraduate Teaching Reform ProjectGuangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine Scientific Research ProjectGuangxi Educational Science Planning Key Projecte70017https://www.ebi.ac.uk/biostudies/studies/S-EPMC12041129biostudies-literatureUnknown19(1)SAE1, a key factor in tumour development, has not been thoroughly examined in pancreatic adenocarcinoma (PAAD), a cancer with high incidence and poor prognosis. We conducted a comprehensive study, integrating mRNA data, immunohistochemistry, CRISPR-modified cell line analysis and single-cell RNA sequencing to assess SAE1's role in PAAD. We also used ChIP-Seq to explore SAE1's transcriptional regulation and analysed clinical data, drug sensitivity and molecular docking models. SAE1 mRNA was significantly overexpressed in PAAD, with a substantial impact on cell proliferation and migration. Functional analyses linked SAE1 to cell cycle and DNA replication pathways, suggesting a role in PAAD development. Our study indicates that SAE1 may promote PAAD through cell cycle pathways, with FOXA1 potIET systems biologySAE1 May Play a Pro-Carcinogenic Role in Pancreatic Adenocarcinoma: A Comprehensive Study Integrating Multiple Pieces of Evidence.PMC120411292022JGA146S2024105982122023Z102022ZJY2791GXZYA202302672025GXNSFAA069660WLXSZX24118GXZYA20230270Yu NChen GLi QLi SDChi BTChen YWu TMeng LJWei DMLi MJChen XJHuang SNYe YPfalseSAE1 May Play a Pro-Carcinogenic Role in Pancreatic Adenocarcinoma: A Comprehensive Study Integrating Multiple Pieces of Evidence.SAE1, a key factor in tumour development, has not been thoroughly examined in pancreatic adenocarcinoma (PAAD), a cancer with high incidence and poor prognosis. We conducted a comprehensive study, integrating mRNA data, immunohistochemistry, CRISPR-modified cell line analysis and single-cell RNA sequencing to assess SAE1's role in PAAD. We also used ChIP-Seq to explore SAE1's transcriptional regulation and analysed clinical data, drug sensitivity and molecular docking models. SAE1 mRNA was significantly overexpressed in PAAD, with a substantial impact on cell proliferation and migration. Functional analyses linked SAE1 to cell cycle and DNA replication pathways, suggesting a role in PAAD development. Our study indicates that SAE1 may promote PAAD through cell cycle pathways, with FOXA1 pot2025-01-01T00:00:00Z2025 Jan-Dec2025-06-25T03:06:03.297Z2025-06-25T03:06:03.297ZS-EPMC120411294030218610.1049/syb2.70017