biostudies-literatureSmith HLNational Institute of Allergy and Infectious DiseasesCancer Research UKHannah’s Willberry Wonder Pony CharityUniversity of SouthamptonNIAID NIH HHSNational Institute of HealthHannah's Willberry Wonder Pony Charity714-725https://www.ebi.ac.uk/biostudies/studies/S-EPMC12041772biostudies-literatureUnknown214(4)Macrophage differentiation, phenotype, and function have been assessed extensively in vitro by predominantly deriving human macrophages from peripheral blood. It is accepted that there are differences between macrophages isolated from different human tissues; however, the importance of anatomical source for in vitro differentiation and characterization is less clear. Here, phenotype and function were evaluated between human macrophages derived from bone marrow or peripheral blood. Macrophages were differentiated by adherence of heterogenous cell populations or CD14 isolation and polarized with IFNγ and LPS or IL-4 and IL-13 for 48 hours before evaluation of phenotype and phagocytic capacity. The presence of stromal cells in bone marrow heterogenous cultures resulted in a reduction in macroJournal of immunology (Baltimore, Md. : 1950)Comparison of human macrophages derived from peripheral blood and bone marrow.PMC12041772201819NIH 1U01AI148153-011166416100001U01 AI148153Foxall RBTeal ELGray JCSmith HLHoppe ADDuriez PJBeers SAKanczler JMfalseComparison of human macrophages derived from peripheral blood and bone marrow.Macrophage differentiation, phenotype, and function have been assessed extensively in vitro by predominantly deriving human macrophages from peripheral blood. It is accepted that there are differences between macrophages isolated from different human tissues; however, the importance of anatomical source for in vitro differentiation and characterization is less clear. Here, phenotype and function were evaluated between human macrophages derived from bone marrow or peripheral blood. Macrophages were differentiated by adherence of heterogenous cell populations or CD14 isolation and polarized with IFNγ and LPS or IL-4 and IL-13 for 48 hours before evaluation of phenotype and phagocytic capacity. The presence of stromal cells in bone marrow heterogenous cultures resulted in a reduction in macro2025-01-01T00:00:00Z2025 Apr2026-05-31T03:11:44.86Z2025-06-25T03:06:12.783ZS-EPMC120417724007309210.1093/jimmun/vkae032