{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(4)"],"submitter":["Barber LJ"],"pubmed_abstract":["The site-selective modification of protein N-termini represents a powerful strategy for producing homogeneous bioconjugates. 2-Pyridinecarboxaldehydes have emerged as a leading reagent class in this area. However, these conjugations suffer from relatively slow rates and a degree of reversibility. In this work, we therefore studied the effects of pyridinecarboxaldehyde functionalization on N-terminal modification. This allowed us to provide insight into the factors governing relative contributions from competing reaction pathways and design criteria for second generation reagents for protein labeling. Importantly, 3-methoxy-2-pyridinecarboxaldehydes were identified as providing both accelerated and more stable protein labeling, enabling further applications of this powerful technology."],"journal":["JACS Au"],"pagination":["1983-1991"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12042018"],"repository":["biostudies-literature"],"pubmed_title":["Effect of Pyridinecarboxaldehyde Functionalization on Reactivity and N-Terminal Protein Modification."],"pmcid":["PMC12042018"],"pubmed_authors":["Stankevich KS","Spicer CD","Barber LJ"],"additional_accession":[]},"is_claimable":false,"name":"Effect of Pyridinecarboxaldehyde Functionalization on Reactivity and N-Terminal Protein Modification.","description":"The site-selective modification of protein N-termini represents a powerful strategy for producing homogeneous bioconjugates. 2-Pyridinecarboxaldehydes have emerged as a leading reagent class in this area. However, these conjugations suffer from relatively slow rates and a degree of reversibility. In this work, we therefore studied the effects of pyridinecarboxaldehyde functionalization on N-terminal modification. This allowed us to provide insight into the factors governing relative contributions from competing reaction pathways and design criteria for second generation reagents for protein labeling. Importantly, 3-methoxy-2-pyridinecarboxaldehydes were identified as providing both accelerated and more stable protein labeling, enabling further applications of this powerful technology.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2025-06-25T03:06:35.932Z","creation":"2025-06-25T03:06:35.932Z"},"accession":"S-EPMC12042018","cross_references":{"pubmed":["40313843"],"doi":["10.1021/jacsau.5c00238"]}}