biostudies-literatureUnknown12Izquierdo MShiga toxin-producing Escherichia coli (STEC) is a major food-borne pathogen causing human diseases ranging from diarrhea to life-threatening complications, mainly in young children. Colonization, virulence, and interactions of STEC strains with human gut microbiota are pivotal during infection but remain poorly described, particularly in children, the most affected population. In this work, we evaluated changes in the microbiota and metabolome composition in the <i>in vitro</i> gut model: Toddler ARtificial COLon (T-ARCOL) infected with EHEC O157:H7 strain EDL 933. Stool samples collected from children with STEC-positive diarrhea and stool from the same children after recovery from the diarrheal episode (<i>n=5</i>) were used to inoculate the T-ARCOL model. STEC colonization was progressiMicrobial cell (Graz, Austria)76-92https://www.ebi.ac.uk/biostudies/studies/S-EPMC12042126biostudies-literatureMicrobiota and metabolome dynamics induced by Shiga toxin-producing <i>E. coli</i> in an <i>in vitro</i> model of an infant's colon.PMC12042126O'Sullivan DUriot OBlanquet-Diot SDurif CFarfan MJEtienne-Mesmin LDenis SIzquierdo MBrun MGahan CGMGallardo PfalseMicrobiota and metabolome dynamics induced by Shiga toxin-producing <i>E. coli</i> in an <i>in vitro</i> model of an infant's colon.Shiga toxin-producing Escherichia coli (STEC) is a major food-borne pathogen causing human diseases ranging from diarrhea to life-threatening complications, mainly in young children. Colonization, virulence, and interactions of STEC strains with human gut microbiota are pivotal during infection but remain poorly described, particularly in children, the most affected population. In this work, we evaluated changes in the microbiota and metabolome composition in the <i>in vitro</i> gut model: Toddler ARtificial COLon (T-ARCOL) infected with EHEC O157:H7 strain EDL 933. Stool samples collected from children with STEC-positive diarrhea and stool from the same children after recovery from the diarrheal episode (<i>n=5</i>) were used to inoculate the T-ARCOL model. STEC colonization was progressi2025-01-01T00:00:00Z20252026-06-01T05:52:10.997Z2025-07-08T03:13:53.282ZS-EPMC120421264030935610.15698/mic2025.04.847