{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Shaver AO"],"funding":["NIEHS NIH HHS","NIH HHS"],"pubmed_abstract":["Caenorhabditis elegans (C. elegans) has been and remains a valuable model organism to study developmental biology, aging, neurobiology, and genetics. The large body of work on C. elegans makes it an ideal candidate to integrate into large-population, whole-animal studies to dissect the complex biological components and their relationships with another organism. In order to use C. elegans in collaborative -omics research, a method is needed to generate large populations of animals where a single sample can be split and assayed across diverse platforms for comparative analyses. Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animals"],"journal":["Journal of visualized experiments : JoVE"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12042146"],"repository":["biostudies-literature"],"pubmed_title":["Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations."],"pmcid":["PMC12042146"],"funding_grant_id":["P40 OD010440","U2C ES030167"],"pubmed_authors":["Shaver AO","Andersen EC","Kirby PS","Edison AS","Gouveia GJ"],"additional_accession":[]},"is_claimable":false,"name":"Culture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.","description":"Caenorhabditis elegans (C. elegans) has been and remains a valuable model organism to study developmental biology, aging, neurobiology, and genetics. The large body of work on C. elegans makes it an ideal candidate to integrate into large-population, whole-animal studies to dissect the complex biological components and their relationships with another organism. In order to use C. elegans in collaborative -omics research, a method is needed to generate large populations of animals where a single sample can be split and assayed across diverse platforms for comparative analyses. Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animals","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 May","modification":"2025-07-13T03:04:28.746Z","creation":"2025-07-13T03:04:28.746Z"},"accession":"S-EPMC12042146","cross_references":{"pubmed":["34028439"],"doi":["10.3791/61453"]}}