biostudies-literatureUnknownShaver AONIEHS NIH HHSNIH HHSCaenorhabditis elegans (C. elegans) has been and remains a valuable model organism to study developmental biology, aging, neurobiology, and genetics. The large body of work on C. elegans makes it an ideal candidate to integrate into large-population, whole-animal studies to dissect the complex biological components and their relationships with another organism. In order to use C. elegans in collaborative -omics research, a method is needed to generate large populations of animals where a single sample can be split and assayed across diverse platforms for comparative analyses. Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animalsJournal of visualized experiments : JoVEhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12042146biostudies-literatureCulture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.PMC12042146P40 OD010440U2C ES030167Shaver AOAndersen ECKirby PSEdison ASGouveia GJfalseCulture and Assay of Large-Scale Mixed-Stage Caenorhabditis elegans Populations.Caenorhabditis elegans (C. elegans) has been and remains a valuable model organism to study developmental biology, aging, neurobiology, and genetics. The large body of work on C. elegans makes it an ideal candidate to integrate into large-population, whole-animal studies to dissect the complex biological components and their relationships with another organism. In order to use C. elegans in collaborative -omics research, a method is needed to generate large populations of animals where a single sample can be split and assayed across diverse platforms for comparative analyses. Here, a method to culture and collect an abundant mixed-stage C. elegans population on a large-scale culture plate (LSCP) and subsequent phenotypic data is presented. This pipeline yields sufficient numbers of animals2021-01-01T00:00:00Z2021 May2025-07-13T03:04:28.746Z2025-07-13T03:04:28.746ZS-EPMC120421463402843910.3791/61453