biostudies-literatureUnknown30(1)Liu M<h4>Background</h4>Bladder cancer (BCa) is the most prevalent malignancy of the urinary system. Aberrant glycosylation, driven by specific glycosyltransferases (GTs), plays a pivotal role in various carcinogenic processes. However, the role of GTs-related glycobiomarkers and their underlying mechanisms in BCa remain poorly understood.<h4>Methods</h4>A diagnostic model based on GTs was constructed and validated using multiple bioinformatics tools. The diagnostic and prognostic value, biological functions and potential targeted drugs were assessed using R packages, K-M plotter and molecular docking. The functional impact and mechanism of ALG3 in BCa were investigated through functional assays, RNA sequencing, immunoprecipitation, and lectin pull down assays.<h4>Results</h4>A diagnostic modelEuropean journal of medical research353https://www.ebi.ac.uk/biostudies/studies/S-EPMC12046919biostudies-literatureɑ1,3-mannosyltransferase promotes the malignant progression of bladder cancer through activating TNF signaling pathway.PMC12046919Li SLiu MZhang JJiang WZheng QYan YZhu Sfalseɑ1,3-mannosyltransferase promotes the malignant progression of bladder cancer through activating TNF signaling pathway.<h4>Background</h4>Bladder cancer (BCa) is the most prevalent malignancy of the urinary system. Aberrant glycosylation, driven by specific glycosyltransferases (GTs), plays a pivotal role in various carcinogenic processes. However, the role of GTs-related glycobiomarkers and their underlying mechanisms in BCa remain poorly understood.<h4>Methods</h4>A diagnostic model based on GTs was constructed and validated using multiple bioinformatics tools. The diagnostic and prognostic value, biological functions and potential targeted drugs were assessed using R packages, K-M plotter and molecular docking. The functional impact and mechanism of ALG3 in BCa were investigated through functional assays, RNA sequencing, immunoprecipitation, and lectin pull down assays.<h4>Results</h4>A diagnostic model2025-01-01T00:00:00Z2025 May2026-05-25T03:09:50.986Z2025-07-08T03:13:44.413ZS-EPMC120469194031270310.1186/s40001-025-02604-5