biostudies-literatureZhang SChina Scholarship CouncilAustrian Science Fund FWFHorizon 2020 Framework Programme555-571https://www.ebi.ac.uk/biostudies/studies/S-EPMC12047074biostudies-literatureUnknown22(3)The nutritional value of wheat grains, particularly their protein and metabolite composition, is a result of the grain-filling process, especially in the endosperm. Here, we employ laser microdissection (LMD) combined with shotgun proteomics and metabolomics to generate a cell type-specific proteome and metabolome inventory of developing wheat endosperm at the early (15 DAA) and late (26 DAA) grain-filling stages. We identified 1803 proteins and 41 metabolites from four different cell types (aleurone (AL), sub-aleurone (SA), starchy endosperm (SE) and endosperm transfer cells (ETCs). Differentially expressed proteins were detected, 67 in the AL, 31 in the SA, 27 in the SE and 50 in the ETCs between these two-time points. Cell-type accumulation of specific SUT and GLUT transporters, sucrosePlant biotechnology journalCell-type proteomic and metabolomic resolution of early and late grain filling stages of wheat endosperm.PMC12047074GA 2020 862‐858I5234201706850037GA 2020 862-858Ramsak ZZang FMohammadi Bazargani MJiang DWeckwerth WZhang SKramml HGhatak AGao SChaturvedi PGruden KVarshney RKfalseCell-type proteomic and metabolomic resolution of early and late grain filling stages of wheat endosperm.The nutritional value of wheat grains, particularly their protein and metabolite composition, is a result of the grain-filling process, especially in the endosperm. Here, we employ laser microdissection (LMD) combined with shotgun proteomics and metabolomics to generate a cell type-specific proteome and metabolome inventory of developing wheat endosperm at the early (15 DAA) and late (26 DAA) grain-filling stages. We identified 1803 proteins and 41 metabolites from four different cell types (aleurone (AL), sub-aleurone (SA), starchy endosperm (SE) and endosperm transfer cells (ETCs). Differentially expressed proteins were detected, 67 in the AL, 31 in the SA, 27 in the SE and 50 in the ETCs between these two-time points. Cell-type accumulation of specific SUT and GLUT transporters, sucrose2024-01-01T00:00:00Z2024 Mar2026-04-23T03:20:28.874Z2025-07-06T03:06:01.182ZS-EPMC120470743805033510.1111/pbi.14203