biostudies-literatureRen PNHLBI NIH HHS260-276https://www.ebi.ac.uk/biostudies/studies/S-EPMC12053597biostudies-literatureUnknown45(2)<h4>Background</h4>Smooth muscle cells (SMCs) of cardiac and neural crest origin contribute to the developing proximal aorta and are linked to disease propensity in adults.<h4>Methods</h4>We analyzed single-cell transcriptomes of aortic SMCs from adult mice to determine basal states and changes after disrupting TGFβ (transforming growth factor-β) signaling necessary for aortic homeostasis.<h4>Results</h4>A minority of Myh11 lineage-marked SMCs differentially expressed genes suggestive of embryological origin. Additional analyses in Nkx2-5 and Wnt1 lineage-marked SMCs derived from cardiac and neural crest progenitors, respectively, showed both lineages contributed to a major common cluster and each lineage to a minor distinct cluster. Common cluster SMCs extended from root to arch, cardiac Arteriosclerosis, thrombosis, and vascular biologyHeterogeneous Cardiac-Derived and Neural Crest-Derived Aortic Smooth Muscle Cells Exhibit Similar Transcriptional Changes After TGFβ Signaling Disruption.PMC12053597R01 HL146723R01 HL152197P01 HL107205Li GLi WHassab AHMTellides GRen PJiang BAssi RfalseHeterogeneous Cardiac-Derived and Neural Crest-Derived Aortic Smooth Muscle Cells Exhibit Similar Transcriptional Changes After TGFβ Signaling Disruption.<h4>Background</h4>Smooth muscle cells (SMCs) of cardiac and neural crest origin contribute to the developing proximal aorta and are linked to disease propensity in adults.<h4>Methods</h4>We analyzed single-cell transcriptomes of aortic SMCs from adult mice to determine basal states and changes after disrupting TGFβ (transforming growth factor-β) signaling necessary for aortic homeostasis.<h4>Results</h4>A minority of Myh11 lineage-marked SMCs differentially expressed genes suggestive of embryological origin. Additional analyses in Nkx2-5 and Wnt1 lineage-marked SMCs derived from cardiac and neural crest progenitors, respectively, showed both lineages contributed to a major common cluster and each lineage to a minor distinct cluster. Common cluster SMCs extended from root to arch, cardiac 2025-01-01T00:00:00Z2025 Feb2026-06-11T05:37:50.027Z2026-06-11T03:08:26.238ZS-EPMC120535973969717210.1161/atvbaha.124.32170610.1161/ATVBAHA.124.321706