{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang H"],"funding":["National Natural Science Foundation of China"],"pagination":["e0007225"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12057350"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["69(5)"],"pubmed_abstract":["Compared with tigecycline-resistant gram-negative bacteria, tigecycline adaptive laboratory evolution (ALE) has been preliminarily performed in <i>Staphylococcus aureus</i>. This study aims to develop higher-level tigecycline-resistant <i>S. aureus</i> mutants (TRSAms) and explore the mechanisms behind decreasing susceptibility to tigecycline. In this study, <i>S. aureus</i> strains were cultured in serial-increasing concentrations of tigecycline and successfully obtained high-level TRSAms. Different phenotypic changes in high-level TRSAms were assessed by growth rate measurement, autolysis assays, mutant frequency determination, and virulence evaluations <i>in vivo</i> and <i>in vitro</i>. The phenotypes of fitness cost showed significant differences in these high-level TRSAms. Whole-geno"],"journal":["Antimicrobial agents and chemotherapy"],"pubmed_title":["Phenotypic and genetic stepwise changes in &lt;i&gt;Staphylococcus aureus&lt;/i&gt; during &lt;i&gt;in vitro&lt;/i&gt; adaptive laboratory evolution under the selective pressure of tigecycline."],"pmcid":["PMC12057350"],"funding_grant_id":["32121004"],"pubmed_authors":["Wan P","Zhu Y","Huang H","Lin W","Zeng Z","Chen Y","Luo X"],"additional_accession":[]},"is_claimable":false,"name":"Phenotypic and genetic stepwise changes in &lt;i&gt;Staphylococcus aureus&lt;/i&gt; during &lt;i&gt;in vitro&lt;/i&gt; adaptive laboratory evolution under the selective pressure of tigecycline.","description":"Compared with tigecycline-resistant gram-negative bacteria, tigecycline adaptive laboratory evolution (ALE) has been preliminarily performed in <i>Staphylococcus aureus</i>. This study aims to develop higher-level tigecycline-resistant <i>S. aureus</i> mutants (TRSAms) and explore the mechanisms behind decreasing susceptibility to tigecycline. In this study, <i>S. aureus</i> strains were cultured in serial-increasing concentrations of tigecycline and successfully obtained high-level TRSAms. Different phenotypic changes in high-level TRSAms were assessed by growth rate measurement, autolysis assays, mutant frequency determination, and virulence evaluations <i>in vivo</i> and <i>in vitro</i>. The phenotypes of fitness cost showed significant differences in these high-level TRSAms. Whole-geno","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-06-02T20:47:36.332Z","creation":"2026-04-20T03:14:27.445Z"},"accession":"S-EPMC12057350","cross_references":{"pubmed":["40135899"],"doi":["10.1128/aac.00072-25"]}}