{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sutherland E"],"funding":["American Society for Mass Spectrometry","Washington Research Foundation","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["5606-5614"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12057629"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(12)"],"pubmed_abstract":["Tandem mass spectrometry (MS/MS) is the gold standard for intact glycopeptide identification, enabling peptide sequence elucidation and site-specific localization of glycan compositions. Beam-type collisional activation is generally sufficient for <i>N-</i>glycopeptides, while electron-driven dissociation is crucial for site localization in <i>O-</i>glycopeptides. Modern glycoproteomic methods often employ multiple dissociation techniques within a single LC-MS/MS analysis, but this approach frequently sacrifices sensitivity when analyzing multiple glycopeptide classes simultaneously. Here we explore the utility of intelligent data acquisition for glycoproteomics through real-time library searching (RTLS) to match oxonium ion patterns for on-the-fly selection of the appropriate dissociation"],"journal":["Journal of proteome research"],"pubmed_title":["Autonomous Dissociation-type Selection for Glycoproteomics Using a Real-Time Library Search."],"pmcid":["PMC12057629"],"funding_grant_id":["R00 GM147304","R00GM147304"],"pubmed_authors":["Russell JH","Bergen D","McAlister GC","Huang J","Huguet R","Zabrouskov V","Barshop WD","Mullen C","Sutherland E","Riley NM","Kothlow K","Canterbury JD","Veth TS"],"additional_accession":[]},"is_claimable":false,"name":"Autonomous Dissociation-type Selection for Glycoproteomics Using a Real-Time Library Search.","description":"Tandem mass spectrometry (MS/MS) is the gold standard for intact glycopeptide identification, enabling peptide sequence elucidation and site-specific localization of glycan compositions. Beam-type collisional activation is generally sufficient for <i>N-</i>glycopeptides, while electron-driven dissociation is crucial for site localization in <i>O-</i>glycopeptides. Modern glycoproteomic methods often employ multiple dissociation techniques within a single LC-MS/MS analysis, but this approach frequently sacrifices sensitivity when analyzing multiple glycopeptide classes simultaneously. Here we explore the utility of intelligent data acquisition for glycoproteomics through real-time library searching (RTLS) to match oxonium ion patterns for on-the-fly selection of the appropriate dissociation","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Dec","modification":"2026-06-10T05:17:21.071Z","creation":"2026-06-10T03:07:19.018Z"},"accession":"S-EPMC12057629","cross_references":{"pubmed":["39531532"],"doi":["10.1021/acs.jproteome.4c00723"]}}