{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bagger SM"],"funding":["German Research Foundation","Vetenskapsrådet","NIMH NIH HHS","National Institutes of Health","Lundbeck Foundation","European Research Council","Kirsten and Freddy Johansens Foundation","Horizon 2020 Framework Programme","Foundation for the National Institutes of Health","Karolinska Institutet","Horizon 2020","Swedish Cancer Society","Minneapolis Medical Research Foundation","Novo Nordisk Fonden","NIGMS NIH HHS"],"pagination":["108441"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12059339"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["301(5)"],"pubmed_abstract":["ADGRA3 (GPR125) is an orphan adhesion G protein-coupled receptor (aGPCR) involved in planar cell polarity, primarily through recruitment of the signaling components disheveled (DVL) during vertebrate gastrulation and discs large homolog 1, implicated in cancer. Limited knowledge exists of the canonical G protein-coupled receptor pathways downstream of ADGRA3. Here, we employed a series of human cell line-based signaling assays to gain insight into the G protein-mediated signaling of ADGRA3. We designed ADGRA3 constructs based on transcript variant analysis in publicly available human liver and brain RNA-seq datasets. Cleavage in the GPCR autoproteolysis site (GPS) is an aGPCR hallmark; thus, we generated a truncated ADGRA3 (C-terminal fragment, CTF) corresponding to a potential cleavage at"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Complex G-protein signaling of the adhesion GPCR, ADGRA3."],"pmcid":["PMC12059339"],"funding_grant_id":["2020-117043","2017-112697","R01 MH054137","GM149539","MH54137","421152132","504098926","20OC0062899","NNF22OC0078104","CRC1423","2019-01190","427840891","NNF20OC0062899","R35 GM149539","682549","R242-2017-409"],"pubmed_authors":["Schihada H","Drzazga AK","Tall GG","Spiess K","Bagger SM","Molleskov-Jensen AS","Rosenkilde MM","Walser ALS","Gratz L","Schoneberg T","Palmisano T","Mathiasen SJ","Mavri M","Schulte G","Kuhn CK","Javitch JA"],"additional_accession":[]},"is_claimable":false,"name":"Complex G-protein signaling of the adhesion GPCR, ADGRA3.","description":"ADGRA3 (GPR125) is an orphan adhesion G protein-coupled receptor (aGPCR) involved in planar cell polarity, primarily through recruitment of the signaling components disheveled (DVL) during vertebrate gastrulation and discs large homolog 1, implicated in cancer. Limited knowledge exists of the canonical G protein-coupled receptor pathways downstream of ADGRA3. Here, we employed a series of human cell line-based signaling assays to gain insight into the G protein-mediated signaling of ADGRA3. We designed ADGRA3 constructs based on transcript variant analysis in publicly available human liver and brain RNA-seq datasets. Cleavage in the GPCR autoproteolysis site (GPS) is an aGPCR hallmark; thus, we generated a truncated ADGRA3 (C-terminal fragment, CTF) corresponding to a potential cleavage at","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-04-20T03:22:50.807Z","creation":"2026-04-20T03:14:36.161Z"},"accession":"S-EPMC12059339","cross_references":{"pubmed":["40127866"],"doi":["10.1016/j.jbc.2025.108441"]}}