{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["32"],"submitter":["Liao CY"],"pubmed_abstract":["In fibrotic tissues, activated fibroblasts remodel the collagen-rich extracellular matrix (ECM). Intervening with this process represents a candidate therapeutic strategy to attenuate disease progression. Models that generate quantitative data on 3D fibroblast-mediated ECM remodeling with the reproducibility and throughput needed for drug testing are lacking. Here, we develop a model that fits this purpose and produces combined quantitative information on drug efficacy and cytotoxicity. We use microinjection robotics to design patterns of fibrillar collagen-embedded fibroblast clusters and apply automated microscopy and image analysis to quantify ECM remodeling between-, and cell viability within clusters of TGFβ-activated primary human skin or lung fibroblasts. We apply this assay to comp"],"journal":["Materials today. Bio"],"pagination":["101800"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12059351"],"repository":["biostudies-literature"],"pubmed_title":["Novel high throughput 3D ECM remodeling assay identifies MEK as key driver of fibrotic fibroblast activity."],"pmcid":["PMC12059351"],"pubmed_authors":["Ten Dijke P","Crawford J","Hundscheid JHM","Danen EHJ","Coornaert B","Liao CY"],"additional_accession":[]},"is_claimable":false,"name":"Novel high throughput 3D ECM remodeling assay identifies MEK as key driver of fibrotic fibroblast activity.","description":"In fibrotic tissues, activated fibroblasts remodel the collagen-rich extracellular matrix (ECM). Intervening with this process represents a candidate therapeutic strategy to attenuate disease progression. Models that generate quantitative data on 3D fibroblast-mediated ECM remodeling with the reproducibility and throughput needed for drug testing are lacking. Here, we develop a model that fits this purpose and produces combined quantitative information on drug efficacy and cytotoxicity. We use microinjection robotics to design patterns of fibrillar collagen-embedded fibroblast clusters and apply automated microscopy and image analysis to quantify ECM remodeling between-, and cell viability within clusters of TGFβ-activated primary human skin or lung fibroblasts. We apply this assay to comp","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-06-01T10:24:39.046Z","creation":"2026-04-08T11:20:05.069Z"},"accession":"S-EPMC12059351","cross_references":{"pubmed":["40343164"],"doi":["10.1016/j.mtbio.2025.101800"]}}