{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Madden PJ"],"funding":["Howard Hughes Medical Institute","NIAID NIH HHS","NIH HHS"],"pagination":["997-1014.e11"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12060733"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(4)"],"pubmed_abstract":["Rare naive B cells have special pathogen-recognition features that enable outsized contributions to protective immunity but infrequently participate in immune responses. We investigatee how germline-targeting vaccine delivery and adjuvant selection affect priming of exceptionally rare BG18-like HIV broadly neutralizing antibody-precursor B cells (<1-in-50 million) in non-human primates. Only escalating dose (ED) priming immunization using the saponin adjuvant SMNP elicited detectable BG18-like cells in germinal centers (GCs) compared with other conditions. All groups had strong GC responses, but only ED+SMNP and bolus+SMNP induced BG18-like memory B cells in >50% of animals. One group had vaccine-specific GC responses equivalent to ED+SMNP but scarce BG18-like B cells. Following homologous"],"journal":["Immunity"],"pubmed_title":["Diverse priming outcomes under conditions of very rare precursor B cells."],"pmcid":["PMC12060733"],"funding_grant_id":["R37 AI125068","S10 OD025052","UM1 AI144462","P51 OD011132","S10 OD026799"],"pubmed_authors":["Marina-Zarate E","Irvine DJ","Schultze S","Georgeson E","Shil M","Maiorino L","Lewis VR","Zhou A","Crotty S","Phelps N","Alavi N","Liguori A","Steichen JM","Rodrigues KA","Schief WR","Pradhan A","Pai Kasturi S","Madden PJ","Michaels KK","Ni K","Melo MB","Lopez PG","Shields K","Lu D","Flynn C","Lemnios A","Xiao S","Kubitz M","Phung I","Bosinger SE","Saha S","Carnathan DG","Smith ML","Lim D","Metz A","Eskandarzadeh S","Gregory J","Rodriguez OL","Healy BS","Ben-Akiva E","Pinney W","Watson CT","Upadhyay AA","Silvestri G","Kalyuzhiny O"],"additional_accession":[]},"is_claimable":false,"name":"Diverse priming outcomes under conditions of very rare precursor B cells.","description":"Rare naive B cells have special pathogen-recognition features that enable outsized contributions to protective immunity but infrequently participate in immune responses. We investigatee how germline-targeting vaccine delivery and adjuvant selection affect priming of exceptionally rare BG18-like HIV broadly neutralizing antibody-precursor B cells (<1-in-50 million) in non-human primates. Only escalating dose (ED) priming immunization using the saponin adjuvant SMNP elicited detectable BG18-like cells in germinal centers (GCs) compared with other conditions. All groups had strong GC responses, but only ED+SMNP and bolus+SMNP induced BG18-like memory B cells in >50% of animals. One group had vaccine-specific GC responses equivalent to ED+SMNP but scarce BG18-like B cells. Following homologous","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-06-02T13:09:49.505Z","creation":"2026-04-18T03:11:23.191Z"},"accession":"S-EPMC12060733","cross_references":{"pubmed":["40168992"],"doi":["10.1016/j.immuni.2025.03.003"]}}