biostudies-literatureZhao YNational Natural Science Funds15987https://www.ebi.ac.uk/biostudies/studies/S-EPMC12062407biostudies-literatureUnknown15(1)JS-K is a precursor drug of nitric oxide (NO) and inhibits tumor growth through various mechanisms. Ferroptosis, a form of cell death closely related to lipid peroxidation, is increasingly being recognized for its role in cancer biology. However, the relevance of ferroptosis in the anti-tumor effects of JS-K is yet to be defined. The cytotoxic effects of erastin and JS-K were evaluated in various renal cell carcinoma (RCC) cell lines and normal human renal epithelial cells. Cell viability and the intracellular levels of ferrous ions, glutathione (GSH), lipid peroxides, and malondialdehyde (MDA) were measured using standard in vitro assays. The expression levels of specific proteins were analyzed by western blotting. Subcutaneous xenografts of RCC were established in a nude mouse model, andScientific reportsJS-K induces ferroptosis in renal carcinoma cells by regulating the c-Myc-GSTP1 Axis.PMC1206240781272833Qiu JLiu JMiu BZhu LLin XLi BZhao YGao SfalseJS-K induces ferroptosis in renal carcinoma cells by regulating the c-Myc-GSTP1 Axis.JS-K is a precursor drug of nitric oxide (NO) and inhibits tumor growth through various mechanisms. Ferroptosis, a form of cell death closely related to lipid peroxidation, is increasingly being recognized for its role in cancer biology. However, the relevance of ferroptosis in the anti-tumor effects of JS-K is yet to be defined. The cytotoxic effects of erastin and JS-K were evaluated in various renal cell carcinoma (RCC) cell lines and normal human renal epithelial cells. Cell viability and the intracellular levels of ferrous ions, glutathione (GSH), lipid peroxides, and malondialdehyde (MDA) were measured using standard in vitro assays. The expression levels of specific proteins were analyzed by western blotting. Subcutaneous xenografts of RCC were established in a nude mouse model, and2025-01-01T00:00:00Z2025 May2026-06-06T16:13:18.268Z2026-06-02T03:11:33.779ZS-EPMC120624074034167710.1038/s41598-025-97887-3