{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Narbonne-Reveau K"],"funding":["Ligue Contre le Cancer","Agence Nationale de la Recherche","NIH HHS"],"pagination":["e1011680"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12064045"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(4)"],"pubmed_abstract":["MicroRNAs are essential regulators of gene expression. Their function is particularly important during neurogenesis, when the production of large numbers of neurons from a limited number of neural stem cells depends on the precise control of determination, proliferation and differentiation. However, microRNAs can target many mRNAs and vice-versa, raising the question of how specificity is achieved to elicit a precise regulatory response. Here we introduce in vivo AGO-APP, a novel approach to purify Argonaute-bound, and therefore active microRNAs from specific cell types. Using AGO-APP in the larval Drosophila central nervous system, we identify a module of microRNAs predicted to redundantly target all iconic genes known to control the transition from neuroblasts to neurons. While microRNA "],"journal":["PLoS genetics"],"pubmed_title":["In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors."],"pmcid":["PMC12064045"],"funding_grant_id":[") (MicroRNAct, ANR-17-CE16-0025; Uncoding, ANR-21-CE16-0034; Miniature, ANR-21-CE13-0003; Goligo, ANR-21-CE16-0023) and the Fondation pour la Recherche Médicale (Program Equipe FRM)","programme Equipe Labellisée","P40 OD018537"],"pubmed_authors":["Erni A","Beclin C","Cremer H","Maurange C","Kapoor S","Narbonne-Reveau K","Eichner N","Sankar S","Meister G"],"additional_accession":[]},"is_claimable":false,"name":"In vivo AGO-APP identifies a module of microRNAs cooperatively preserving neural progenitors.","description":"MicroRNAs are essential regulators of gene expression. Their function is particularly important during neurogenesis, when the production of large numbers of neurons from a limited number of neural stem cells depends on the precise control of determination, proliferation and differentiation. However, microRNAs can target many mRNAs and vice-versa, raising the question of how specificity is achieved to elicit a precise regulatory response. Here we introduce in vivo AGO-APP, a novel approach to purify Argonaute-bound, and therefore active microRNAs from specific cell types. Using AGO-APP in the larval Drosophila central nervous system, we identify a module of microRNAs predicted to redundantly target all iconic genes known to control the transition from neuroblasts to neurons. While microRNA ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-07T03:16:02.465Z","creation":"2026-07-07T03:08:30.532Z"},"accession":"S-EPMC12064045","cross_references":{"pubmed":["40299997"],"doi":["10.1371/journal.pgen.1011680"]}}