biostudies-literatureKotnis SNIAID NIH HHSThis work was supported by funds provided by a National Science Foundation (NSF) grant to Q. S. (2203715), USA; NSF Emerging Frontiers in Research and Innovation (EFRI) to Q. S. (2132156), USA; Texas A&M Excellence Fund X-grants to Q. S., Texas A&M, USA; Texas A&M Targeted Proposal Teams (TPT) grant to Q. S., Texas A&M, USA; NIH NIAID R01 to Q. S. (R01AI165433), Texas A&M, USA. This work was partially supported by the National Science Foundation (NSF) under Award No. 2330245, which funds the Engineering Research Center for Carbon Utilization Redesign through Biomanufacturing-Empowered Decarbonization (CURB).1397-1401https://www.ebi.ac.uk/biostudies/studies/S-EPMC12067030biostudies-literatureUnknown122(6)The widespread consumption of PET worldwide has necessitated the search for environment-friendly methods for PET degradation and recycling. Among these methods, biodegradation stands out as a promising approach for recycling PET. The discovery of duo enzyme system PETase and MHETase in 2016, along with their engineered variants, has demonstrated significant potential in breaking down PET. Previous studies have also demonstrated that the activity of the enzyme PETase increases when it is immobilized on nanoparticles. To achieve highly efficient and complete PET depolymerization, we immobilized both FAST-PETase and MHETase at a specific ratio on magnetic nanoparticles. This immobilization resulted in a 2.5-fold increase in product release compared with free enzymes. Additionally, we achievedBiotechnology and bioengineeringHigh-Efficiency PET Degradation With a Duo-Enzyme System Immobilized on Magnetic Nanoparticles.PMC12067030R01 AI165433Sun QKotnis SGulati SfalseHigh-Efficiency PET Degradation With a Duo-Enzyme System Immobilized on Magnetic Nanoparticles.The widespread consumption of PET worldwide has necessitated the search for environment-friendly methods for PET degradation and recycling. Among these methods, biodegradation stands out as a promising approach for recycling PET. The discovery of duo enzyme system PETase and MHETase in 2016, along with their engineered variants, has demonstrated significant potential in breaking down PET. Previous studies have also demonstrated that the activity of the enzyme PETase increases when it is immobilized on nanoparticles. To achieve highly efficient and complete PET depolymerization, we immobilized both FAST-PETase and MHETase at a specific ratio on magnetic nanoparticles. This immobilization resulted in a 2.5-fold increase in product release compared with free enzymes. Additionally, we achieved2025-01-01T00:00:00Z2025 Jun2026-07-15T05:43:04.939Z2026-07-01T03:07:10.113ZS-EPMC120670304006270010.1002/bit.28963