{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Auddino S"],"funding":["F-CUR, University of Siena","Italian Ministry of University and Research","Bando Ricerca Finalizzata 2018,  Italian Ministry of Health","Italian Ministry of University and Research (MUR) PNRR \"National Center for Gene Therapy and Drugs based on RNA Technology\" , CN3 Spoke #5 \"Inflammatory and Infectious Diseases\"","Innovative Medicines Initiative 2 (IMI2) Joint Undertaking","Bando Ricerca Finalizzata 2018, Italian Ministry of Health","Italian Ministry of University and Research (MUR) PNRR “National Center for Gene Therapy and Drugs based on RNA Technology” , CN3 Spoke #5 “Inflammatory and Infectious Diseases”","Università degli Studi di Siena"],"pagination":["1261-1278"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12069488"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["68(6)"],"pubmed_abstract":["<h4>Aims/hypothesis</h4>MiRNAs regulate gene expression, influencing beta cell function and pathways. Isoforms of miRNA (isomiRs), sequence variants of miRNAs with post-transcriptional modifications, exhibit cell-type-specific expression and functions. Despite their biological significance, a comprehensive isomiR profile in human pancreatic islets and beta cells remains unexplored. This study aims to profile isomiR expression in four beta cell sources: (1) laser capture microdissected human islets (LCM-HI); (2) collagenase-isolated human islets (CI-HI); (3) sorted beta cells; and (4) the EndoC-βH1 beta cell line, and to investigate their potential role in beta cell function.<h4>Methods</h4>Small RNA-seq and/or small RNA dataset analysis was conducted on human pancreatic islets and beta cel"],"journal":["Diabetologia"],"pubmed_title":["Comprehensive sequencing profile and functional analysis of IsomiRs in human pancreatic islets and beta cells."],"pmcid":["PMC12069488"],"funding_grant_id":["115797 INNODIA","Project No. CN00000041","945268 INNODIA HARVEST","2017KAM2R5_003","2268-2022-SG-PSR2021-FCUR_001","GR-2018-12365577"],"pubmed_authors":["Tondolo V","Quero G","Sebastiani G","Pedace E","Grieco GE","Fignani D","Po A","Berteramo AF","Licata G","Mori A","Aiello E","Guay C","Auddino S","Mezza T","Regazzi R","Soldovieri L","Dotta F","Nigi L","Giaccari A","Formichi C","Di Giuseppe G","Ciccarelli G","Mari A","Bruttini M"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive sequencing profile and functional analysis of IsomiRs in human pancreatic islets and beta cells.","description":"<h4>Aims/hypothesis</h4>MiRNAs regulate gene expression, influencing beta cell function and pathways. Isoforms of miRNA (isomiRs), sequence variants of miRNAs with post-transcriptional modifications, exhibit cell-type-specific expression and functions. Despite their biological significance, a comprehensive isomiR profile in human pancreatic islets and beta cells remains unexplored. This study aims to profile isomiR expression in four beta cell sources: (1) laser capture microdissected human islets (LCM-HI); (2) collagenase-isolated human islets (CI-HI); (3) sorted beta cells; and (4) the EndoC-βH1 beta cell line, and to investigate their potential role in beta cell function.<h4>Methods</h4>Small RNA-seq and/or small RNA dataset analysis was conducted on human pancreatic islets and beta cel","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jun","modification":"2026-06-02T20:47:47.329Z","creation":"2026-04-20T03:14:29.402Z"},"accession":"S-EPMC12069488","cross_references":{"pubmed":["40102237"],"doi":["10.1007/s00125-025-06397-4"]}}