biostudies-literatureAuddino SF-CUR, University of SienaItalian Ministry of University and ResearchBando Ricerca Finalizzata 2018, Italian Ministry of HealthItalian Ministry of University and Research (MUR) PNRR "National Center for Gene Therapy and Drugs based on RNA Technology" , CN3 Spoke #5 "Inflammatory and Infectious Diseases"Innovative Medicines Initiative 2 (IMI2) Joint UndertakingBando Ricerca Finalizzata 2018, Italian Ministry of HealthItalian Ministry of University and Research (MUR) PNRR “National Center for Gene Therapy and Drugs based on RNA Technology” , CN3 Spoke #5 “Inflammatory and Infectious Diseases”Università degli Studi di Siena1261-1278https://www.ebi.ac.uk/biostudies/studies/S-EPMC12069488biostudies-literatureUnknown68(6)<h4>Aims/hypothesis</h4>MiRNAs regulate gene expression, influencing beta cell function and pathways. Isoforms of miRNA (isomiRs), sequence variants of miRNAs with post-transcriptional modifications, exhibit cell-type-specific expression and functions. Despite their biological significance, a comprehensive isomiR profile in human pancreatic islets and beta cells remains unexplored. This study aims to profile isomiR expression in four beta cell sources: (1) laser capture microdissected human islets (LCM-HI); (2) collagenase-isolated human islets (CI-HI); (3) sorted beta cells; and (4) the EndoC-βH1 beta cell line, and to investigate their potential role in beta cell function.<h4>Methods</h4>Small RNA-seq and/or small RNA dataset analysis was conducted on human pancreatic islets and beta celDiabetologiaComprehensive sequencing profile and functional analysis of IsomiRs in human pancreatic islets and beta cells.PMC12069488115797 INNODIAProject No. CN00000041945268 INNODIA HARVEST2017KAM2R5_0032268-2022-SG-PSR2021-FCUR_001GR-2018-12365577Tondolo VQuero GSebastiani GPedace EGrieco GEFignani DPo ABerteramo AFLicata GMori AAiello EGuay CAuddino SMezza TRegazzi RSoldovieri LDotta FNigi LGiaccari AFormichi CDi Giuseppe GCiccarelli GMari ABruttini MfalseComprehensive sequencing profile and functional analysis of IsomiRs in human pancreatic islets and beta cells.<h4>Aims/hypothesis</h4>MiRNAs regulate gene expression, influencing beta cell function and pathways. Isoforms of miRNA (isomiRs), sequence variants of miRNAs with post-transcriptional modifications, exhibit cell-type-specific expression and functions. Despite their biological significance, a comprehensive isomiR profile in human pancreatic islets and beta cells remains unexplored. This study aims to profile isomiR expression in four beta cell sources: (1) laser capture microdissected human islets (LCM-HI); (2) collagenase-isolated human islets (CI-HI); (3) sorted beta cells; and (4) the EndoC-βH1 beta cell line, and to investigate their potential role in beta cell function.<h4>Methods</h4>Small RNA-seq and/or small RNA dataset analysis was conducted on human pancreatic islets and beta cel2025-01-01T00:00:00Z2025 Jun2026-06-02T20:47:47.329Z2026-04-20T03:14:29.402ZS-EPMC120694884010223710.1007/s00125-025-06397-4