biostudies-literatureLi XNational Natural Science Funds of China4274https://www.ebi.ac.uk/biostudies/studies/S-EPMC12072129biostudies-literatureUnknown26(9)The endoplasmic reticulum-Golgi apparatus system is an important organelle regulating male reproduction. USO1 vesicle transport factor (USO1), as an important molecule in this system, is a general vesicular transport factor and regulates various biological processes in vivo. However, the potential role of USO1 in mammalian testis development and spermatogenesis has not been investigated. We documented the presence of USO1 in mouse germ cells and its functional roles by generating <i>Uso1</i>-knockout germ cell lines. <i>Uso1</i> depletion suppressed cell proliferation and growth while stimulating apoptosis in GC1 and GC2 cells. In addition, the <i>Uso1</i> knockout blocked cell cycle progression and weakened DNA damage repair. Mechanistically, USO1 is associated with male reproduction by regulating the expression of genes involved in spermatogenesis, and we found evidence that USO1 is closely linked to centriolar satellites (CSs), which may play an important biological role. Overall, our findings reveal a vital role for USO1 in male fertility and offer a significant understanding of the functions of golgin proteins in reproductive biology.International journal of molecular sciencesUSO1 Coordinates Centriolar Satellites to Regulate Male Germ Cell Proliferation and Cell Cycle Progression.PMC1207212932270834Li XLin PJiang ZXie YZhang ZWang RFeng XCai JXu SfalseUSO1 Coordinates Centriolar Satellites to Regulate Male Germ Cell Proliferation and Cell Cycle Progression.The endoplasmic reticulum-Golgi apparatus system is an important organelle regulating male reproduction. USO1 vesicle transport factor (USO1), as an important molecule in this system, is a general vesicular transport factor and regulates various biological processes in vivo. However, the potential role of USO1 in mammalian testis development and spermatogenesis has not been investigated. We documented the presence of USO1 in mouse germ cells and its functional roles by generating <i>Uso1</i>-knockout germ cell lines. <i>Uso1</i> depletion suppressed cell proliferation and growth while stimulating apoptosis in GC1 and GC2 cells. In addition, the <i>Uso1</i> knockout blocked cell cycle progression and weakened DNA damage repair. Mechanistically, USO1 is associated with male reproduction by regulating the expression of genes involved in spermatogenesis, and we found evidence that USO1 is closely linked to centriolar satellites (CSs), which may play an important biological role. Overall, our findings reveal a vital role for USO1 in male fertility and offer a significant understanding of the functions of golgin proteins in reproductive biology.2025-01-01T00:00:00Z2025 Apr2026-04-08T15:48:48.414Z2026-04-08T05:34:46.939ZS-EPMC120721294036251510.3390/ijms26094274