biostudies-literatureKlymus KStrategic Environmental Research and Development ProgramUSGS Ecosystems Mission AreaGreat Lakes Restoration InitiativeU.S. Fish and Wildlife ServiceU.S. Department of Defense430-436https://www.ebi.ac.uk/biostudies/studies/S-EPMC12077482biostudies-literatureUnknown10(6)Freshwater mussels of the Unionida order are important to freshwater ecosystems but are highly imperiled worldwide. Improving our understanding of these species is crucial to their continued conservation. Some Unionid mussels exhibit double uniparental inheritance (DUI) in which individuals have two mitochondrial genomes. Of those species with DUI, sequences of the female mitotype are most prevalent in genetic databases. Here, we demonstrate the ability to recover both mitotypes of Ortmanniana ligamentina (Lamarck, 1819) from a non-lethal collection method coupled with high-throughput sequencing. Increased male mitotype sequence representation facilitates understanding Unionid genetic diversity and development of molecular tools for species detection.Mitochondrial DNA. Part B, ResourcesThe complete mitochondrial genomes of the freshwater mussel <i>Ortmanniana ligamentina</i> (Lamarck, 1819): male and female mitotypes.PMC12077482SERDP RC-19-1156Klymus KRuiz-Ramos DBarnhart CCoombs JMaloy AfalseThe complete mitochondrial genomes of the freshwater mussel <i>Ortmanniana ligamentina</i> (Lamarck, 1819): male and female mitotypes.Freshwater mussels of the Unionida order are important to freshwater ecosystems but are highly imperiled worldwide. Improving our understanding of these species is crucial to their continued conservation. Some Unionid mussels exhibit double uniparental inheritance (DUI) in which individuals have two mitochondrial genomes. Of those species with DUI, sequences of the female mitotype are most prevalent in genetic databases. Here, we demonstrate the ability to recover both mitotypes of Ortmanniana ligamentina (Lamarck, 1819) from a non-lethal collection method coupled with high-throughput sequencing. Increased male mitotype sequence representation facilitates understanding Unionid genetic diversity and development of molecular tools for species detection.2025-01-01T00:00:00Z20252026-06-01T05:56:22.991Z2026-04-08T09:41:06.698ZS-EPMC120774824037112810.1080/23802359.2025.2500528