biostudies-literatureUnknown75(5)Cheng GSvenska Forskningsrådet FormasEuropean Research CouncilA strictly anaerobic bacterial strain, designated as AMB_02^T, was isolated from a propionate enrichment culture obtained from a high-ammonia biogas digester. The cells were anaerobic and coccoid (0.5 µm), often appearing as diplococci or in a short chain of three to four cells. Growth was observed between 20 and 45 °C (optimum at 37-39 °C), with an initial pH of 6.5-9.0 (optimum pH 8.0-8.5), and the species tolerated up to 0.8 M NH₄Cl and 0.5 M NaCl. The major cellular fatty acids were C_16 : 0 (31.6%), C_14 : 0 (14.6%) and C_18 : 0 (13.3%). AMB_02^T grew with formate, carbohydrates and aa, including asparagine, histidine, tryptone and tryptophan. Acetate was the major product formed. Phylogenetic analysis based on 16S rRNA geneInternational journal of systematic and evolutionary microbiologyhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC12079837biostudies-literature<i>Microaceticoccus formicicus</i> gen. nov., sp. nov., an ammonia-tolerant formate-utilizing bacterium originating from a biogas process.PMC120798379481382018-01341Schnurer ACheng GWesterholm Mfalse<i>Microaceticoccus formicicus</i> gen. nov., sp. nov., an ammonia-tolerant formate-utilizing bacterium originating from a biogas process.A strictly anaerobic bacterial strain, designated as AMB_02^T, was isolated from a propionate enrichment culture obtained from a high-ammonia biogas digester. The cells were anaerobic and coccoid (0.5 µm), often appearing as diplococci or in a short chain of three to four cells. Growth was observed between 20 and 45 °C (optimum at 37-39 °C), with an initial pH of 6.5-9.0 (optimum pH 8.0-8.5), and the species tolerated up to 0.8 M NH₄Cl and 0.5 M NaCl. The major cellular fatty acids were C_16 : 0 (31.6%), C_14 : 0 (14.6%) and C_18 : 0 (13.3%). AMB_02^T grew with formate, carbohydrates and aa, including asparagine, histidine, tryptone and tryptophan. Acetate was the major product formed. Phylogenetic analysis based on 16S rRNA gene2025-01-01T00:00:00Z2025 May2026-06-03T02:47:21.577Z2026-04-23T03:11:55.681ZS-EPMC120798374033864110.1099/ijsem.0.006773